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Literature summary for 1.13.11.2 extracted from

  • Wei, J.; Zhou, Y.; Xu, T.; Lu, B.
    Rational design of catechol-2, 3-dioxygenase for improving the enzyme characteristics (2009), Appl. Biochem. Biotechnol., 162, 116-126.
    View publication on PubMed
Search for more literature for 1.13.11.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Pseudomonas sp.

Protein Variants

Protein Variants Comment Organism
A229C forming disulfide bonds, more alkalescency stable, improvement of thermostability, widened optimum temperature from 40-50°C Pseudomonas sp.
A229C virtually mutated enzyme Pseudomonas putida
H294C forming disulfide bonds, more alkalescency stable, improvement of thermostability, widened optimum temperature from 40-50°C Pseudomonas sp.
H294C virtually mutated enzyme Pseudomonas putida
additional information used for identifying potential sites of introducing disulfide bond of C23O from Pseudomonas sp. Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 catechol wild type C23O Pseudomonas sp.
0.04
-
 catechol mutated C23O: A229C, H294C Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P06622 mt-2, used for identifying potential sites of introducing disulfide bond of C23O from Pseudomonas sp.
-
Pseudomonas sp.
-
-
-
Pseudomonas sp. CGMCC2953
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA-agarose column Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
300
-
 highest specific activity at arount 40°C for both wild-type and mutated C23O Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2 assay at pH 7.5, 30°C Pseudomonas sp. ?
-
 ?
catechol + O2 assay at pH 7.5, 30°C Pseudomonas sp. CGMCC2953 ?
-
 ?

Synonyms

Synonyms Comment Organism
C23o
-
 Pseudomonas sp.
C23o
-
 Pseudomonas putida
catechol-2,3-dioxygenase
-
 Pseudomonas sp.
catechol-2,3-dioxygenase
-
 Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Pseudomonas sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 wild-type C23O only 45% residue activity at 50°C, mutated C23O still 90% residue activity at 50°C Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 38% relative activity of the wild-type enzyme after 1200 min at 30°C, 45% relative activity of mutated enzymes after 1200 min at 30°C, 44% relative activity of wild-type enzyme after 80 min at 50°C, 52% relative activity of mutated enzyme after 80 min at 50°C Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Pseudomonas sp.

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5 wild-type C23O only 50% residue activity at pH 9.5, mutated C23O still 75% residue activity at pH 9.5 Pseudomonas sp.