Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
General Stability | Organism |
---|---|
25°C, half-life of 69 min in air, 37°C, half-life of 70 min in argon | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Pseudomonas putida mt2 | - |
- |
- |
Oxidation Stability | Organism |
---|---|
25°C, half-life of 69 min in air, 37°C, half-life of 70 min in argon | Pseudomonas putida |
Purification (Comment) | Organism |
---|---|
recombinant protein | Pseudomonas putida |
Renatured (Comment) | Organism |
---|---|
inactivation of XylE enzyme by 4-methylcatechol results in oxidation of the active site iron to a high spin ferric state. Soluble [2Fe-2S] ferredoxin protein XylT reactivates XylE through reduction of the iron atom in the active site of the enzyme. XylE reactivation involves catalytic nonstoichiometric amounts of XylT | Pseudomonas putida |
Subunits | Comment | Organism |
---|---|---|
More | interaction of inactive enzyme with soluble [2Fe-2S] ferredoxin protein XylT for reactivation through reduction of the iron atom in the active site of the enzyme | Pseudomonas putida |
Synonyms | Comment | Organism |
---|---|---|
XylE | - |
Pseudomonas putida |