General Stability | Organism |
---|---|
the purified enzyme is unstable losing the activity completely at 8°C within 3 days | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
Tricine SDS-PAGE | Pseudomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | Pseudomonas sp. | catechol 2,3-dioxygenase is involved in the meta phenol degradation pathway | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | Pseudomonas sp. phDV1 | catechol 2,3-dioxygenase is involved in the meta phenol degradation pathway | 2-hydroxymuconate semialdehyde | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. phDV1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
50 mM Tris-SO4 buffer, pH 8.0, at 4 °C. Sucrose density gradient, two-step anion exchange chromatography using MonoQ column attached at a Dionex HPLC system. | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20 | - |
activity is measured following the formation of 2-hydroxymuconate semialdehyde at 375 nm and 25°C. | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + O2 | catechol 2,3-dioxygenase is involved in the meta phenol degradation pathway | Pseudomonas sp. | 2-hydroxymuconate semialdehyde | - |
? | |
catechol + O2 | catechol 2,3-dioxygenase is involved in the meta phenol degradation pathway | Pseudomonas sp. phDV1 | 2-hydroxymuconate semialdehyde | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C23o | - |
Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | isoelectric focusing | - |
5 |