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Literature summary for 1.13.11.2 extracted from

  • Tsirogianni, E.; Aivaliotis, M.; Karas, M.; Tsiotis, G.
    Detection and characterisation of catechol 2,3-dioxygenase in an indigenous soil pseudomonad by MALDI-TOF MS using a column separation (2005), Biodegradation, 16, 181-186.
    View publication on PubMed

General Stability

General Stability Organism
the purified enzyme is unstable losing the activity completely at 8°C within 3 days Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
Tricine SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Pseudomonas sp. catechol 2,3-dioxygenase is involved in the meta phenol degradation pathway 2-hydroxymuconate semialdehyde
-
?
catechol + O2 Pseudomonas sp. phDV1 catechol 2,3-dioxygenase is involved in the meta phenol degradation pathway 2-hydroxymuconate semialdehyde
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. phDV1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
50 mM Tris-SO4 buffer, pH 8.0, at 4 °C. Sucrose density gradient, two-step anion exchange chromatography using MonoQ column attached at a Dionex HPLC system. Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
activity is measured following the formation of 2-hydroxymuconate semialdehyde at 375 nm and 25°C. Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2 catechol 2,3-dioxygenase is involved in the meta phenol degradation pathway Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
catechol + O2 catechol 2,3-dioxygenase is involved in the meta phenol degradation pathway Pseudomonas sp. phDV1 2-hydroxymuconate semialdehyde
-
?

Synonyms

Synonyms Comment Organism
C23o
-
Pseudomonas sp.

pI Value

Organism Comment pI Value Maximum pI Value
Pseudomonas sp. isoelectric focusing
-
5