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Literature summary for 1.13.11.17 extracted from
- Purification and characterization of a new indole oxygenase from the leaves of Tecoma stans L (1983), Plant Physiol., 71, 19-23.
General Stability
| General Stability | Organism |
|---|---|
| dialysis, 8 h, results in complete loss of activity | Tecoma stans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| azide | 0.5 mM, 54% inhibition | Tecoma stans |  |
| CO | 0.5 mM, 58% inhibition | Tecoma stans | |
| Cu2+ | 1 mM, CuSO4, 25% inhibition | Tecoma stans | |
| cyanide | 0.5 mM, 64% inhibition | Tecoma stans | |
| Fe2+ | 1 mM, FeSO4, 50% inhibition | Tecoma stans | |
| Fe3+ | - |
Tecoma stans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0189 | - |
indole | - |
Tecoma stans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Tecoma stans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Tecoma stans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| indole + O2 | Tecoma stans | during daytime activity is less, it reaches a peak during nighttime | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tecoma stans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Tecoma stans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Tecoma stans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0157 | - |
disappearance of 1 nmol indole in 1 min | Tecoma stans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 18 h without appreciable loss of activity, complete loss of activity after 48 h | Tecoma stans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-bromoindole + O2 | 15% of the activity with indole | Tecoma stans | 5-bromo-2-formylaminobenzaldehyde | - |
? | |
| 5-hydroxyindole + O2 | 20% of the activity with indole | Tecoma stans | 2-formylamino-5-hydroxybenzaldehyde | - |
? | |
| 5-methylindole + O2 | 26% of the activity with indole | Tecoma stans | 2-formylamino-5-methylbenzaldehyde | - |
? | |
| indole + O2 | conversion of indole to anthranilic acid | Tecoma stans | anthranilic acid | - |
? | |
| indole + O2 | during daytime activity is less, it reaches a peak during nighttime | Tecoma stans | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Tecoma stans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Tecoma stans |
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