General Stability | Organism |
---|---|
activity stable in crude extract, inactive apoenzyme stable, can be re-activated by treatment with Fe2+ and ascorbate, irreversible apoenzyme activity loss if stored in completely Fe2+-free buffer, re-activated holoenzyme unstable | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Escherichia coli | |
H2O2 | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.026 | - |
3-(2,3-dihydroxyphenyl)propionate | - |
Escherichia coli | |
0.09 | - |
3-methylcatechol | - |
Escherichia coli | |
0.72 | - |
catechol | - |
Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | weakly bound, 1 mol per mol tetramer, by addition of Fe2+ re-activated apoenzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
4 * 36000, SDS-PAGE | Escherichia coli |
134000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-(2,3-dihydroxyphenyl)propionate + O2 | Escherichia coli | - |
2-hydroxy-6-oxonona-2,4-diene-1,9-dioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABR9 | gene mhpB | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
47.9 | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxyphenylpropionic acid + O2 | - |
Escherichia coli | 2-hydroxy-6-oxonona-dienoate | - |
? | |
3-(2,3-dihydroxyphenyl)propionate + O2 | - |
Escherichia coli | 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate | - |
? | |
3-methylcatechol + O2 | meta-ring cleavage | Escherichia coli | 2-hydroxy-6-oxohepta-2,4-dienoate | - |
? | |
catechol + O2 | meta-ring-cleavage | Escherichia coli | 2-hydroxy-6-oxohexa-2,4-dienoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 36000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
catechol | - |
Escherichia coli | |
3.6 | - |
3-methylcatechol | - |
Escherichia coli | |
29 | - |
3-(2,3-dihydroxyphenyl)propionate | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | - |
Escherichia coli |