Cloned (Comment) | Organism |
---|---|
isoenzyme Oep1LOX2 and Oep2LOX2 | Olea europaea |
Oep1LOX2 and Oep2LOX2 ligated into vector pQE-80 L and overexpressed in Escherichia coli strain XL1-Blue | Olea europaea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits Oep2LOX2 by 49% | Olea europaea | |
Fe3+ | slight inactivation | Olea europaea | |
Hg2+ | inhibits Oep1LOX2 by 60% and totally inactivates Oep2LOX2 | Olea europaea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01459 | - |
linoleic acid | isoform Oep1LOX2 | Olea europaea | |
0.0146 | - |
linoleate | pH 6.5, 20°C, isoenzyme Oep1LOX2 | Olea europaea | |
0.028 | - |
linolenic acid | isoform Oep1LOX2 | Olea europaea | |
0.028 | - |
alpha-linolenate | pH 6.5, 20°C, isoenzyme Oep1LOX2 | Olea europaea | |
0.1297 | - |
linoleic acid | isoform Oep2LOX2 | Olea europaea | |
0.13 | - |
linoleate | pH 6.5, 20°C, isoenzyme Oep2LOX2 | Olea europaea | |
0.1389 | - |
linolenic acid | isoform Oep2LOX2 | Olea europaea | |
0.14 | - |
alpha-linolenate | pH 6.5, 20°C, isoenzyme Oep2LOX2 | Olea europaea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Olea europaea | 9507 | - |
additional information | isoenzyme Oep1LOX2 and Oep2LOX2 contain an N-terminal chloroplastic transit peptide | Olea europaea | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | among the conserved amino acids in sequences of both isozymes are three His, one Asn, and one Ile residue, which are essential for the binding of the atom of iron in the active site of the LOXs enzymes | Olea europaea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101700 | - |
sequence analysis, isoform Oep2LOX2 | Olea europaea |
103400 | - |
sequence analysis, isoform Oep1LOX2 | Olea europaea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Olea europaea | - |
- |
- |
Olea europaea | - |
cv. Picual | - |
Purification (Comment) | Organism |
---|---|
by affinity chromatography | Olea europaea |
isoenzyme Oep1LOX2 and Oep2LOX2 | Olea europaea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Olea europaea | - |
leaf | Oep1LOX2 predominantely expressed | Olea europaea | - |
mesocarp | Oep2LOX2 shows the highest expression levels in mature mesocarp tissue | Olea europaea | - |
seed | Oep2LOX2 during development and ripening of Picual and Arbequina olive fruit | Olea europaea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-linolenate + O2 | isoenzyme Oep1LOX2 and Oep2LOX2 both produce primarily 13-hydroperoxides from linoleic acid and linolenic acid. Linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid) | Olea europaea | (9Z,11E,15Z)-13-hydroperoxy-9,11,15-octadecatrienoate | - |
? | |
linoleate + O2 | isoenzyme Oep1LOX2 and Oep2LOX2 both produce primarily 13-hydroperoxides from linoleic acid and linolenic acid. Linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid) | Olea europaea | (9Z,11E)-13-hydroperoxy-9,11-octadecadienoate | - |
? | |
linoleic acid + O2 | preferred substrate | Olea europaea | (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoic acid | - |
? | |
linolenic acid + O2 | - |
Olea europaea | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
13-lipoxygenase | - |
Olea europaea |
LOX2 | - |
Olea europaea |
Oep1LOX2 | - |
Olea europaea |
Oep2LOX2 | - |
Olea europaea |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
isoenzyme Oep2LOX2 | Olea europaea |
35 | - |
isoform Oep2LOX2 | Olea europaea |
45 | - |
isoenzyme Oep1LOX2 | Olea europaea |
45 | - |
isoform Oep1LOX2 | Olea europaea |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 55 | more than 85% of maximal activity in the temperature-range: 15-55°C, isoenzyme Oep1LOX2 | Olea europaea |
15 | 55 | recombinant Oep1LOX2 exhibits more than 85% of its maximal activity, whereas Oep2LOX2 displays about 50-100% of its maximal activity | Olea europaea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.25 | - |
isoform Oep1LOX2 | Olea europaea |
6.3 | - |
isoenzyme Oep2LOX2 | Olea europaea |
6.75 | - |
isoform Oep2LOX2 | Olea europaea |
6.8 | - |
isoenzyme Oep1LOX2 | Olea europaea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Olea europaea | sequence analysis, isoform Oep2LOX2 | - |
5.9 |
Olea europaea | sequence analysis, isoform Oep1LOX2 | - |
7.2 |
Organism | Comment | Expression |
---|---|---|
Olea europaea | Oep2LOX2 transcript levels increase in Picual and Arbequina mesocarps during development and ripening, showing a maximum at turning stage, whereas Oep1LOX2 exhibits constant expression levels in both olive varieties and tissues | up |
General Information | Comment | Organism |
---|---|---|
physiological function | major involvement of the Oep2LOX2 gene in the biosynthesis of virgin olive oil aroma compounds | Olea europaea |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | linolenic acid is the preferred substrate for both isoenzymes (Vmax/KM is about 10fold higher for linolenic acid) | Olea europaea |