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Literature summary for 1.13.11.1 extracted from

  • Long, Y.; Yang, S.; Xie, Z.; Cheng, L.
    Cloning, expression, and characterization of catechol 1,2-dioxygenase from a phenol-degrading Candida tropicalis JH8 strain (2016), Prep. Biochem. Biotechnol., 46, 673-678 .
    View publication on PubMed
Search for more literature for 1.13.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Candida tropicalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0092
-
 catechol at pH 7.5 and 30°C Candida tropicalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
catechol + O2 Candida tropicalis
-
 cis,cis-muconate
-
 ?
catechol + O2 Candida tropicalis JH8
-
 cis,cis-muconate
-
 ?

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
-
-
Candida tropicalis JH8
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + O2
-
 Candida tropicalis cis,cis-muconate
-
 ?
catechol + O2
-
 Candida tropicalis JH8 cis,cis-muconate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 32000, SDS-PAGE Candida tropicalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Candida tropicalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 recombinant enzyme is unstable below pH 5.0 and stable from pH 7.0 to 9.0 Candida tropicalis