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Literature summary for 1.13.11.1 extracted from

  • Micalella, C.; Martignon, S.; Bruno, S.; Pioselli, B.; Caglio, R.; Valetti, F.; Pessione, E.; Giunta, C.; Rizzi, M.
    X-ray crystallography, mass spectrometry and single crystal microspectrophotometry: A multidisciplinary characterization of catechol 1,2 dioxygenase (2011), Biochim. Biophys. Acta, 1814, 817-823.
    View publication on PubMed
Search for more literature for 1.13.11.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acinetobacter radioresistens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 2 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.5, at 4°C Acinetobacter radioresistens

Protein Variants

Protein Variants Comment Organism
A72G the mutant shows decreased activity with catechol and no activity with 3-methylcatechol compared to the wild type enzyme Acinetobacter radioresistens
L69A the mutant shows decreased activity with catechol and increased activity for 4-chlorocatechol compared to the wild type enzyme Acinetobacter radioresistens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
 4-Chlorocatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.00198
-
 4-Chlorocatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.00204
-
 catechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.00225
-
 3-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.00576
-
 catechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.00595
-
 3-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.0061
-
 4-Chlorocatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.0093
-
 catechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.01
-
 4-methylcatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.0128
-
 4-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.053
-
 4-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens

Metals/Ions

Metals/Ions Comment Organism Structure
Fe3+ Fe3+-dependent enzyme Acinetobacter radioresistens

Organism

Organism UniProt Comment Textmining
Acinetobacter radioresistens Q9F103
-
-
Acinetobacter radioresistens LMG S13 Q9F103
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, gel filtration Acinetobacter radioresistens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
3-methylcatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens LMG S13 (2Z,4Z)-2-methylhexa-2,4-dienedioate
-
 ?
4,5-dichlorocatechol + O2 the enzyme possesses only a quite rare capability to process 4,5-dichlorocatechol Acinetobacter radioresistens ?
-
 ?
4,5-dichlorocatechol + O2 the enzyme possesses only a quite rare capability to process 4,5-dichlorocatechol Acinetobacter radioresistens LMG S13 ?
-
 ?
4-chlorocatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
 ?
4-chlorocatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens LMG S13 (2E,4Z)-3-chlorohexa-2,4-dienedioate
-
 ?
4-methylcatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens 4-methyl-cis,cis-muconate
-
 ?
4-methylcatechol + O2 the enzyme preferentially recognizes 4-substituted over 3-substituted catechols Acinetobacter radioresistens LMG S13 4-methyl-cis,cis-muconate
-
 ?
catechol + O2 best substrate Acinetobacter radioresistens cis,cis-muconate
-
 ?
catechol + O2 best substrate Acinetobacter radioresistens LMG S13 cis,cis-muconate
-
 ?

Synonyms

Synonyms Comment Organism
1,2 CTD
-
 Acinetobacter radioresistens
catechol 1,2 dioxygenase
-
 Acinetobacter radioresistens
IsoB
-
 Acinetobacter radioresistens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.17
-
 4,5-dichlorocatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.17
-
 4,5-dichlorocatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.18
-
 4,5-dichlorocatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.34
-
 4-Chlorocatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.52
-
 3-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.72
-
 4-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
0.83
-
 4-Chlorocatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.59
-
 3-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1.74
-
 4-Chlorocatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
4.42
-
 catechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
5.45
-
 4-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
5.51
-
 4-methylcatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
13.6
-
 catechol mutant enzyme A72G, in100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
24.2
-
 catechol wild type enzyme, in100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
280
-
 4-Chlorocatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
550
-
 4-methylcatechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
570
-
 4-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
880
-
 3-methylcatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1000
-
 4-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
1460
-
 catechol mutant enzyme A72G, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
3440
-
 4-Chlorocatechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
4200
-
 4-Chlorocatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
7100
-
 3-methylcatechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
7800
-
 catechol mutant enzyme L69A, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens
11800
-
 catechol wild type enzyme, in 100 mM Tris-HCl pH 8.5, temperature not specified in the publication Acinetobacter radioresistens