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Literature summary for 1.13.11.1 extracted from

  • Giedraityte, G.; Kalediene, L.
    Catechol 1,2-dioxygenase from alpha-naphthol degrading thermophilic Geobacillus sp. strain: Purification and properties (2009), Cent. Eur. J. Biol., 4, 68-73.
No PubMed abstract available
Search for more literature for 1.13.11.1

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 0.1 mM, 10 min, complete inhibition Geobacillus sp.
CuSO4 0.1 mM, 10 min, complete inhibition Geobacillus sp.
EDTA 1 mM, 10 min, 20% inhibition Geobacillus sp.
FeSO4 0.1 mM, 10 min, 12% inhibition Geobacillus sp.
H2O2 0.1 mM, 10 min, 98% inhibition Geobacillus sp.
mercaptoethanol 0.1 mM, 10 min, 98% inhibition Geobacillus sp.
o-phenanthroline 0.05 mM, 10 min, 3% inhibition Geobacillus sp.
streptomycin sulfate 5%, 10 min, 80% inhibition Geobacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 catechol
-
 Geobacillus sp.
0.286
-
 3-methylcatechol
-
 Geobacillus sp.
0.4
-
 4-methylcatechol
-
 Geobacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
 SDS-PAGE Geobacillus sp.

Organism

Organism UniProt Comment Textmining
Geobacillus sp.
-
-
-
Geobacillus sp. G27
-
-
-

Purification (Commentary)

Purification (Comment) Organism
about 18fold after ammonium sulfate precipitation, DEAE-celullose, Sephadex G-150 and hydroxylapatite chromatographie Geobacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.42
-
-
 Geobacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-methylcatechol + O2 assay at 60°C, pH 7.0 Geobacillus sp. ?
-
 ?
3-methylcatechol + O2 assay at 60°C, pH 7.0 Geobacillus sp. G27 ?
-
 ?
4-methylcatechol + O2 assay at 60°C, pH 7.0 Geobacillus sp. ?
-
 ?
4-methylcatechol + O2 assay at 60°C, pH 7.0 Geobacillus sp. G27 ?
-
 ?
catechol + O2 intradiol, assay at 60°C, pH 7.0 Geobacillus sp. cis,cis-muconate
-
 ?
catechol + O2 intradiol, assay at 60°C, pH 7.0 Geobacillus sp. G27 cis,cis-muconate
-
 ?

Synonyms

Synonyms Comment Organism
C1,2O
-
 Geobacillus sp.
catechol 1,2-dioxygenase
-
 Geobacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Geobacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 90 only 3% enzyme activity lost at 50-70°C, at 80°C 22% lose of 22% of enzyme activity, below 50°C and 70°C rapidly decreased enzyme activity Geobacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 2 month: 100% enzyme activity left Geobacillus sp.
60
-
 half-life: 40 min Geobacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Geobacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 11 45% of specific activity in pH range from 8-10.5 Geobacillus sp.