Cloned (Comment) | Organism |
---|---|
gene catA, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic tree, identification of the cat gene cluster of strain MT1 | Pseudomonas reinekei |
gene salD, DNA and amino acid sequence determination and analysis, genetic organization, phylogenetic tree | Pseudomonas reinekei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0008 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.001 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.001 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0017 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0056 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0075 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0099 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, SDS-PAGE, x * 34233, sequence calculation | Pseudomonas reinekei |
34209 | - |
x * 34209, sequence calculation | Pseudomonas reinekei |
34233 | - |
x * 33000, SDS-PAGE, x * 34233, sequence calculation | Pseudomonas reinekei |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylcatechol + O2 | Pseudomonas reinekei | - |
(2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | Pseudomonas reinekei | - |
3-methyl-cis,cis-hexadienedioate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas reinekei | A0F0B5 | gene cat; encoded by the cat gene cluster, gene catA | - |
Pseudomonas reinekei | Q0VH42 | gene salD; encoded by the cat gene cluster, gene catA | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:5-chlorosalicylate-grown cell | - |
Pseudomonas reinekei | - |
culture condition:salicylate-grown cell | - |
Pseudomonas reinekei | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
pH not specified in the publication, 25°C, substrate 4-chlorocatechol | Pseudomonas reinekei |
1.4 | - |
pH not specified in the publication, 25°C, substrate 3-methylcatechol | Pseudomonas reinekei |
2.9 | - |
pH not specified in the publication, 25°C, substrate 4-chlorocatechol | Pseudomonas reinekei |
3.8 | - |
pH not specified in the publication, 25°C, substrate 3-methylcatechol | Pseudomonas reinekei |
4.4 | - |
pH not specified in the publication, 25°C, substrate 4-methylcatechol | Pseudomonas reinekei |
12.3 | - |
pH not specified in the publication, 25°C, substrate catechol | Pseudomonas reinekei |
15.3 | - |
pH not specified in the publication, 25°C, substrate catechol | Pseudomonas reinekei |
35.8 | - |
pH not specified in the publication, 25°C, substrate 4-methylcatechol | Pseudomonas reinekei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
Pseudomonas reinekei | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
4-chlorocatechol + O2 | - |
Pseudomonas reinekei | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas reinekei | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | preferred substrate | Pseudomonas reinekei | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | - |
Pseudomonas reinekei | 3-methyl-cis,cis-hexadienedioate | - |
? | |
catechol + O2 | - |
Pseudomonas reinekei | cis,cis-muconate | - |
? | |
additional information | no activity with 3-chlorocatechol | Pseudomonas reinekei | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33000, SDS-PAGE, x * 34233, sequence calculation | Pseudomonas reinekei |
? | x * 34209, sequence calculation | Pseudomonas reinekei |
Synonyms | Comment | Organism |
---|---|---|
C12O | - |
Pseudomonas reinekei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.8 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
1.7 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
2.3 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
2.7 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
7.5 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
8.7 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
20.7 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei |
General Information | Comment | Organism |
---|---|---|
metabolism | 4- and 5-methylsalicylate as growth substrates are effectively converted by enzymes of the sal cluster into 4-methylmuconolactone, followed by isomerization to 3-methylmuconolactone. The function of the sal gene cluster is therefore to channel both chloro-substituted and methyl-substituted salicylates into a catechol ortho cleavage pathway, followed by dismantling of the formed substituted muconolactones through specific pathways | Pseudomonas reinekei |
additional information | strain MT1 possesses two catechol 1,2-dioxygenases, encoded by the genes catA and salD, overview. The enzymes differ in their turnover rates for 4-chlorocatechol and especially for 4-methylcatechol, where C12OsalD exhibit significantly higher rates. Both enzymes also differ with respect to their apparent Km values | Pseudomonas reinekei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0003 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0004 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0007 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0008 | - |
3-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.001 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0017 | - |
4-Chlorocatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0109 | - |
catechol | pH not specified in the publication, 25°C | Pseudomonas reinekei | |
0.0122 | - |
4-methylcatechol | pH not specified in the publication, 25°C | Pseudomonas reinekei |