Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 1 mM, 52% inhibition | Rhodococcus rhodochrous | |
PCMB | 0.1 mM, 16% inhibition | Rhodococcus rhodochrous | |
Sodium dithionite | 1 mM, 36% loss of activity | Rhodococcus rhodochrous | |
Tiron | 0.5 mM, 20% loss of activity | Rhodococcus rhodochrous |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Rhodococcus rhodochrous |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Rhodococcus rhodochrous |
71000 | - |
gel filtration | Rhodococcus rhodochrous |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus rhodochrous | - |
- |
- |
Rhodococcus rhodochrous N75 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus rhodochrous |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dimethylcatechol + O2 | 21% of activity with catechol | Rhodococcus rhodochrous | 3,4-dimethyl-cis,cis-muconate | - |
? | |
3,4-dimethylcatechol + O2 | 21% of activity with catechol | Rhodococcus rhodochrous N75 | 3,4-dimethyl-cis,cis-muconate | - |
? | |
3,5-dimethylcatechol + O2 | 36% of activity with catechol | Rhodococcus rhodochrous | 3,5-dimethyl-cis,cis-muconate | - |
? | |
3,5-dimethylcatechol + O2 | 36% of activity with catechol | Rhodococcus rhodochrous N75 | 3,5-dimethyl-cis,cis-muconate | - |
? | |
3-methylcatechol + O2 | 64% of activity with catechol | Rhodococcus rhodochrous | 2-methyl-cis,cis-muconate | - |
? | |
3-methylcatechol + O2 | 64% of activity with catechol | Rhodococcus rhodochrous N75 | 2-methyl-cis,cis-muconate | - |
? | |
4-chlorocatechol + O2 | - |
Rhodococcus rhodochrous | 4-chloro-cis,cis-muconate | - |
? | |
4-ethylcatechol + O2 | 42% of activity with catechol | Rhodococcus rhodochrous | 4-ethyl-cis-cis-muconate | - |
? | |
4-fluorocatechol + O2 | 65% of activity with catechol | Rhodococcus rhodochrous | 4-fluoro-cis,cis-muconate | - |
? | |
4-iso-propylcatechol + O2 | 23% of activity with catechol | Rhodococcus rhodochrous | 4-isopropyl-cis,cis-muconate | - |
? | |
4-methylcatechol + O2 | 76% of activity with catechol | Rhodococcus rhodochrous | 3-methyl-cis,cis-muconate | - |
? | |
4-methylcatechol + O2 | 76% of activity with catechol | Rhodococcus rhodochrous N75 | 3-methyl-cis,cis-muconate | - |
? | |
4-n-propylcatechol + O2 | 17% of activity with catechol | Rhodococcus rhodochrous | 4-n-propyl-cis,cis-muconate | - |
? | |
catechol + O2 | - |
Rhodococcus rhodochrous | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Rhodococcus rhodochrous N75 | cis,cis-muconate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Rhodococcus rhodochrous |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life of the enzyme from benzoate-grown cells is 27 min, half-life of the enzyme from p-toluate-grown cells is 14 min | Rhodococcus rhodochrous |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
in 50 mM Bis-Tris-propane buffer and 50 mM Tris-HCl buffer | Rhodococcus rhodochrous |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | high activity | Rhodococcus rhodochrous |