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Literature summary for 1.13.11.1 extracted from

  • Tsai, S.C.; Li, Y.K.
    Purification and characterization of a catechol 1,2-dioxygenase from a phenol degrading Candida albicans TL3 (2007), Arch. Microbiol., 187, 199-206.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 0.001 mM, 93% loss of activity Candida albicans
CuCl2 0.1 mM, 41% inhibition Candida albicans
FeCl3 0.1 mM, 14% inhibition Candida albicans
HgCl2 0.1 mM, 64% inhibition Candida albicans
PbSO4 0.1 mM, 75% loss of activity Candida albicans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0093
-
catechol 25°C Candida albicans
0.0215
-
4-methylcatechol 25°C Candida albicans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe each subunit contained one iron Candida albicans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
2 * 32000, SDS-PAGE Candida albicans
64000
-
gel filtration Candida albicans

Organism

Organism UniProt Comment Textmining
Candida albicans
-
-
-
Candida albicans TL3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Candida albicans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
63
-
-
Candida albicans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
Candida albicans 3-methyl-cis,cis-muconate
-
?
4-methylcatechol + O2
-
Candida albicans TL3 3-methyl-cis,cis-muconate
-
?
catechol + O2
-
Candida albicans cis,cis-muconate
-
?
catechol + O2
-
Candida albicans TL3 cis,cis-muconate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 32000, SDS-PAGE Candida albicans

Synonyms

Synonyms Comment Organism
1,2-CTD
-
Candida albicans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
-
Candida albicans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 8.3, 30 min, stable below Candida albicans
50
-
pH 8.3, 30 min, about 35% of maximal activity Candida albicans
60
-
pH 8.3, 30 min, about 85% of maximal activity Candida albicans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 catechol 25°C Candida albicans
5.6
-
4-methylcatechol 25°C Candida albicans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Candida albicans

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 pH 6.0: about 60% of maximal activity, pH 9.5: about 60% of maximal activity Candida albicans

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 9 30 min, maintaining more than 85% activity Candida albicans