Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-dichlorocatechol + O2 | Cupriavidus necator | reaction catalyzed by catechol 1,2-dioxygenase type II | (2E,4E)-2,4-dichlorohexa-2,4-dienedioate | - |
? | |
3,5-dichlorocatechol + O2 | Cupriavidus necator JMP 134-1 | reaction catalyzed by catechol 1,2-dioxygenase type II | (2E,4E)-2,4-dichlorohexa-2,4-dienedioate | - |
? | |
3-chloro-5-methylcatechol + O2 | Cupriavidus necator | reaction catalyzed by catechol 1,2-dioxygenase type II | 2-chloro-4-methylhexa-2,4-dienedioate | - |
? | |
3-chloro-5-methylcatechol + O2 | Cupriavidus necator JMP 134-1 | reaction catalyzed by catechol 1,2-dioxygenase type II | 2-chloro-4-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | Cupriavidus necator | reaction catalyzed by catechol 1,2-dioxygenase type I and type II | 3-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | Cupriavidus necator JMP 134-1 | reaction catalyzed by catechol 1,2-dioxygenase type I and type II | 3-methylhexa-2,4-dienedioate | - |
? | |
catechol + O2 | Cupriavidus necator | reaction catalyzed by catechol 1,2-dioxygenase type I | cis,cis-muconate | - |
? | |
catechol + O2 | Cupriavidus necator JMP 134-1 | reaction catalyzed by catechol 1,2-dioxygenase type I | cis,cis-muconate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
a spontaneous mutant of strain JMP 134, expressing the enzymes 2,4-D monooxygenase, chlorophenol hydroxylase, catechol 1,2-dioxygenase, dichloromuconate cycloisomerase, dienelactone hydrolase, and maleylacetate reductase constitutively | - |
Cupriavidus necator JMP 134-1 | - |
a spontaneous mutant of strain JMP 134, expressing the enzymes 2,4-D monooxygenase, chlorophenol hydroxylase, catechol 1,2-dioxygenase, dichloromuconate cycloisomerase, dienelactone hydrolase, and maleylacetate reductase constitutively | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | 2-chloro-4-methylphenoxyacetate is no growth substrate for Alcaligenes eutrophus JMP 134 andJMP 134-1 | Cupriavidus necator | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.01 | - |
whole cell induced by D-fructose, pH 7.4, 30°C | Cupriavidus necator |
0.19 | - |
whole cell induced by 2,4-dichlorophenoxyacetate, pH 7.4, 30°C | Cupriavidus necator |
0.41 | - |
whole cell induced by 2-chloro-4-methylphenoxyacetic acid, pH 7.4, 30°C | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,5-dichlorocatechol + O2 | reaction catalyzed by catechol 1,2-dioxygenase type II | Cupriavidus necator | (2E,4E)-2,4-dichlorohexa-2,4-dienedioate | - |
? | |
3,5-dichlorocatechol + O2 | reaction catalyzed by catechol 1,2-dioxygenase type II | Cupriavidus necator JMP 134-1 | (2E,4E)-2,4-dichlorohexa-2,4-dienedioate | - |
? | |
3-chloro-5-methylcatechol + O2 | reaction catalyzed by catechol 1,2-dioxygenase type II | Cupriavidus necator | 2-chloro-4-methylhexa-2,4-dienedioate | - |
? | |
3-chloro-5-methylcatechol + O2 | reaction catalyzed by catechol 1,2-dioxygenase type II | Cupriavidus necator JMP 134-1 | 2-chloro-4-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | reaction catalyzed by catechol 1,2-dioxygenase type I and type II | Cupriavidus necator | 3-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | reaction catalyzed by catechol 1,2-dioxygenase type I and type II | Cupriavidus necator JMP 134-1 | 3-methylhexa-2,4-dienedioate | - |
? | |
catechol + O2 | reaction catalyzed by catechol 1,2-dioxygenase type I | Cupriavidus necator | cis,cis-muconate | - |
? | |
catechol + O2 | reaction catalyzed by catechol 1,2-dioxygenase type I | Cupriavidus necator JMP 134-1 | cis,cis-muconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
catechol 1,2-dioxygenase type I | - |
Cupriavidus necator |
catechol 1,2-dioxygenase type II | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in vivo assay at | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
in vivo assay at | Cupriavidus necator |
Organism | Comment | Expression |
---|---|---|
Cupriavidus necator | 2,4-dichlorophenoxyacetate and 2-chloro-4-methylphenoxyacetate induce the enzyme, 2-chloro-4-methylphenoxyacetate induces the enzymes of the chloroaromatic pathway, i.e. 2,4-D monooxygenase, chlorophenol hydroxylase, catechol 1,2-dioxygenase type II, dichloromuconate cycloisomerase, dienelactone hydrolase and maleylacetate reductase | up |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of the chloroaromatic pathway and catalyzes the third step of the cometabolism of 2-chloro-4-methylphenoxyacetate by Alcaligenes eutrophus JMP 134 after growth on 2,4-dichlorophenoxyacetate, overview | Cupriavidus necator |