KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
catechol | - |
Acinetobacter radioresistens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 0.96 mol per mol of protein | Acinetobacter radioresistens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
78000 | 79000 | PAGE, gel filtration | Acinetobacter radioresistens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter radioresistens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Acinetobacter radioresistens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.5 | - |
- |
Acinetobacter radioresistens |
Storage Stability | Organism |
---|---|
4°C, 100% activity after 1 month | Acinetobacter radioresistens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
Acinetobacter radioresistens | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | - |
Acinetobacter radioresistens | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
catechol + O2 | - |
Acinetobacter radioresistens | cis,cis-muconate | - |
? | |
additional information | both intradiol and extradiol cleavage of 3-methylcatechol | Acinetobacter radioresistens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38700-39000, at low ionic strength, upon increasing the ionic strength the enzyme monomerizes, SDS-PAGE | Acinetobacter radioresistens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 47 | - |
Acinetobacter radioresistens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.8 | - |
catechol | - |
Acinetobacter radioresistens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | the activity rapidly decreases outside this range | Acinetobacter radioresistens |