Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | the large subunit HoxC is purified without its small subunit. Two forms of HoxC are identified. Both forms contain iron but only substoichiometric amounts of nickel. One form is a homodimer of HoxC whereas the second also contains the NiFe site maturation proteins HypC and HypB. Despite the presence of the NiFe active site in some of the proteins, both forms, which lack the FeS clusters normally present in hydrogenases, cannot activate hydrogen. The incomplete insertion of nickel into the NiFe site provides direct evidence that Fe precedes Ni in the course of metal center assembly | Cupriavidus necator |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Purification (Comment) | Organism |
---|---|
the large subunit HoxC is purified without its small subunit. Two forms of HoxC are identified. Both forms contain iron but only substoichiometric amounts of nickel. One form is a homodimer of HoxC whereas the second also contains the Ni-Fe site maturation proteins HypC and HypB. Despite the presence of the Ni-Fe active site in some of the proteins, both forms, which lack the Fe-S clusters normally present in hydrogenases, cannot activate hydrogen. The incomplete insertion of nickel into the NiFe site provides direct evidence that Fe precedes Ni in the course of metal center assembly | Cupriavidus necator |
Synonyms | Comment | Organism |
---|---|---|
H2-sensing [NiFe] hydrogenase | - |
Cupriavidus necator |