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Literature summary for 1.12.99.6 extracted from

  • Zadvorny, O.A.; Zorin, N.A.; Gogotov, I.N.; Gorlenko, V.M.
    Properties of stable hydrogenase from the purple sulfur bacterium Lamprobacter modestohalophilus (2004), Biochemistry, 69, 164-169.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cd2+ reversible inhibition Lamprobacter modestohalophilus
CO noncompetitive vs. oxidized methyl viologen, competitive vs. H2 Lamprobacter modestohalophilus
Mg2+ reversible inhibition Lamprobacter modestohalophilus
Ni2+ competitive vs. Oxidized methyl viologen Lamprobacter modestohalophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
Lamprobacter modestohalophilus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
1 * 64000 + 1 * 30000, SDS-PAGE Lamprobacter modestohalophilus
64000
-
1 * 64000 + 1 * 30000, SDS-PAGE Lamprobacter modestohalophilus

Organism

Organism UniProt Comment Textmining
Lamprobacter modestohalophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lamprobacter modestohalophilus

Storage Stability

Storage Stability Organism
20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under air, 50 d, approx. 80% loss of activity Lamprobacter modestohalophilus
20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under argon, 50 d, approx. 60% loss of activity Lamprobacter modestohalophilus
20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under H2, 50 d, approx. 70% loss of activity Lamprobacter modestohalophilus
4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under air, 60 d, approx. 40% loss of activity Lamprobacter modestohalophilus
4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under argon, 60 d, approx. 30% loss of activity Lamprobacter modestohalophilus
4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under H2, 60 d, approx. 50% loss of activity Lamprobacter modestohalophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + oxidized benzyl viologen
-
Lamprobacter modestohalophilus reduced benzyl viologen + H+
-
r
H2 + oxidized methyl viologen
-
Lamprobacter modestohalophilus reduced methyl viologen + H+
-
r

Subunits

Subunits Comment Organism
dimer 1 * 64000 + 1 * 30000, SDS-PAGE Lamprobacter modestohalophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
-
Lamprobacter modestohalophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 95 almost no activity at 20°C, approx. 20% of maximal activity at 95°C Lamprobacter modestohalophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
no loss of activity after 1.5 min at pH 7.0 Lamprobacter modestohalophilus
85
-
approx. 50% loss of activity after 1.5 min at pH 7.0 Lamprobacter modestohalophilus
100
-
almost complete loss of activity after 1.5 min at pH 7.0 Lamprobacter modestohalophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
H2 evolution Lamprobacter modestohalophilus
8.5 9.5 H2 oxidation Lamprobacter modestohalophilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.007
-
CO 30°C, pH 7.0 Lamprobacter modestohalophilus
0.16
-
Ni2+ 30°C, pH 9.0 Lamprobacter modestohalophilus
1.35
-
Ni2+ 30°C, pH 7.0 Lamprobacter modestohalophilus

pI Value

Organism Comment pI Value Maximum pI Value
Lamprobacter modestohalophilus isoelectric focusing
-
4.5