Literature summary for 1.12.99.6 extracted from

Meuer, J.; Bartoschek, S.; Koch, J.; Kunkel, A.; Hedderich, R.
Purification and catalytic properties of Ech hydrogenase from Methanosarcina barkeri (1999), Eur. J. Biochem., 265, 325-335.

Search for more literature for 1.12.99.6

Inhibitors

Inhibitors	Comment	Organism	Structure
CO	50% inhibition at 7.5% CO in the gas phase	Methanosarcina barkeri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.001	-	oxidized ferredoxin	-	Methanosarcina barkeri
0.005	-	H2	-	Methanosarcina barkeri
0.0075	-	reduced ferredoxin	-	Methanosarcina barkeri

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	11.3 mol per mol of enzyme	Methanosarcina barkeri
Ni	0.9 mol per mol of enzyme	Methanosarcina barkeri
Sulfide	10.8 mol acid labile sulfur per mol of enzyme	Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
H2 + acceptor	Methanosarcina barkeri	ferredoxin acts as physiological electron donor	H+ + reduced acceptor	-	?
H2 + acceptor	Methanosarcina barkeri Fusaro / DSM 804	ferredoxin acts as physiological electron donor	H+ + reduced acceptor	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanosarcina barkeri	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
apparent homogeneity	Methanosarcina barkeri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
50	-	reduction of ferredoxin	Methanosarcina barkeri
90	-	H2 formation with reduced ferredoxin	Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + acceptor	benzyl viologen, methylene blue, ferredoxin and methylviologen can act as electron acceptors	Methanosarcina barkeri	H+ + reduced acceptor	-	?
H2 + acceptor	ferredoxin acts as physiological electron donor	Methanosarcina barkeri	H+ + reduced acceptor	-	?
H2 + acceptor	benzyl viologen, methylene blue, ferredoxin and methylviologen can act as electron acceptors	Methanosarcina barkeri Fusaro / DSM 804	H+ + reduced acceptor	-	?
H2 + acceptor	ferredoxin acts as physiological electron donor	Methanosarcina barkeri Fusaro / DSM 804	H+ + reduced acceptor	-	?
H2 + oxidized ferredoxin	-	Methanosarcina barkeri	reduced ferredoxin	-	r
H2 + oxidized ferredoxin	-	Methanosarcina barkeri Fusaro / DSM 804	reduced ferredoxin	-	r
reduced ferredoxin	-	Methanosarcina barkeri	H2 + oxidized ferredoxin	-	r
reduced ferredoxin	-	Methanosarcina barkeri Fusaro / DSM 804	H2 + oxidized ferredoxin	-	r

Subunits

Subunits	Comment	Organism
hexamer	6 different subunits A, B, C, D, E, and F, SDS-PAGE	Methanosarcina barkeri

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Methanosarcina barkeri

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Release 2023.1 (January 2023)