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Literature summary for 1.12.99.6 extracted from

  • Hatchikian, E.C.; Forget, N.; Fernandez, V.M.; Williams, R.; Cammack, R.
    Further characterization of the [iron]-hydrogenase from Desulfovibrio desulfuricans ATCC 7757 (1992), Eur. J. Biochem., 209, 357-365.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
CO
-
Desulfovibrio desulfuricans
Cu2+ 65% loss of activity after 2 min at 0.005 mM, complete inhibition at 0.1 mM in the presence of ascorbate, no inhibition observed without ascorbate Desulfovibrio desulfuricans
diethyldicarbonate 65% loss of activity 6 mM Desulfovibrio desulfuricans
nitrite 70% loss of activity after 2 min at 0.04 mM Desulfovibrio desulfuricans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe 14 atoms per molecule, 2 4Fe-4S clusters Desulfovibrio desulfuricans
Sulfide 14.4 atoms per molecule Desulfovibrio desulfuricans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
1 * 42500 + 1 * 11000, SDS-PAGE Desulfovibrio desulfuricans
42500
-
1 * 42500 + 1 * 11000, SDS-PAGE Desulfovibrio desulfuricans
53500
-
analytical ultracentrifugation Desulfovibrio desulfuricans

Organism

Organism UniProt Comment Textmining
Desulfovibrio desulfuricans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Desulfovibrio desulfuricans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activities with various electron donors Desulfovibrio desulfuricans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + acceptor methyl viologen acts as electron acceptor Desulfovibrio desulfuricans H+ + reduced acceptor
-
r

Subunits

Subunits Comment Organism
dimer 1 * 42500 + 1 * 11000, SDS-PAGE Desulfovibrio desulfuricans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00016
-
CO
-
Desulfovibrio desulfuricans