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Literature summary for 1.12.99.6 extracted from
- Partial purification and characterization of the first hydrogenase isolated from a thermophilic sulfate-reducing bacterium (1992), Biochem. Biophys. Res. Commun., 184, 1256-1260.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NO | - |
Thermodesulfobacterium thermophilum |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 7-8 atoms per mol enzyme, 3Fe-4S cluster | Thermodesulfobacterium thermophilum | |
| Ni | 0.6-0.7 atoms per mol enzyme | Thermodesulfobacterium thermophilum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 15000 | - |
1 * 55000 + 1 * 15000, SDS-PAGE | Thermodesulfobacterium thermophilum |
| 55000 | - |
1 * 55000 + 1 * 15000, SDS-PAGE | Thermodesulfobacterium thermophilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermodesulfobacterium thermophilum | - |
strain DSM 1276 formerly Desulfovibrio thermophilus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Thermodesulfobacterium thermophilum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 783 | - |
H2 uptake | Thermodesulfobacterium thermophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + acceptor | benzyl viologen acts as electron acceptor | Thermodesulfobacterium thermophilum | H+ + reduced acceptor | - |
? | |
| H2 + acceptor | methyl viologen acts as electron acceptor | Thermodesulfobacterium thermophilum | H+ + reduced acceptor | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 55000 + 1 * 15000, SDS-PAGE | Thermodesulfobacterium thermophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermodesulfobacterium thermophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
H2 production | Thermodesulfobacterium thermophilum |
| 7.8 | - |
H2 uptake | Thermodesulfobacterium thermophilum |
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