Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | reversible | Pyrococcus furiosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
H2 | with methylene blue as electron acceptor | Pyrococcus furiosus | |
1.9 | - |
H2 | with methyl viologen as electron acceptor | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 31 g iron per 185 g enzyme | Pyrococcus furiosus | |
Ni | 31 g nickel per 185 g enzyme | Pyrococcus furiosus | |
Sulfide | 24 g acid labile sulfide per 185 g enzyme | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
27000 | - |
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
46000 | - |
alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
185000 | - |
gel filtration | Pyrococcus furiosus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + acceptor | Pyrococcus furiosus | ferredoxin acts as physiological electron donor | H+ + reduced acceptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Purification (Comment) | Organism |
---|---|
apparent homogeneity | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
360 | - |
- |
Pyrococcus furiosus |
Storage Stability | Organism |
---|---|
-196°C no loss of activity after freezing and thawing under argon | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + acceptor | methyl viologen acts as electron acceptor | Pyrococcus furiosus | H+ + reduced acceptor | - |
r | |
H2 + acceptor | methylene blue acts as electron acceptor | Pyrococcus furiosus | H+ + reduced acceptor | - |
r | |
H2 + acceptor | reduced ferredoxin can act as acceptor | Pyrococcus furiosus | H+ + reduced acceptor | - |
r | |
H2 + acceptor | ferredoxin acts as physiological electron donor | Pyrococcus furiosus | H+ + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha2,beta2,gamma2, 2 * 46000 + 2 * 27000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
above | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
no loss of activity after 2 months under anaerobic conditions | Pyrococcus furiosus |
80 | - |
50% loss of activity after 23 h | Pyrococcus furiosus |
90 | - |
50% loss of activity after 3 h | Pyrococcus furiosus |
100 | - |
50% loss of activity after 2 h | Pyrococcus furiosus |
105 | - |
50% loss of activity after 5 min | Pyrococcus furiosus |