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Literature summary for 1.12.98.4 extracted from

  • Dirmeier, R.; Keller, M.; Frey, G.; Huber, H.; Stetter, K.O.
    Purification and properties of an extremely thermostable membrane-bound sulfur-reducing complex from the hyperthermophilic Pyrodictium abyssi (1998), Eur. J. Biochem., 252, 486-491.
    View publication on PubMed
Search for more literature for 1.12.98.4

Activating Compound

Activating Compound Comment Organism Structure
additional information no quinione, but enzyme complex contains cytochrome c and has two additional cytochromes, probably of b-type Pyrodictium abyssi

Inhibitors

Inhibitors Comment Organism Structure
2-(n-heptyl)-4-hydroxyquinoline N-oxide not affected by Pyrodictium abyssi

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Pyrodictium abyssi 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu 1.2 mol per mol protein Pyrodictium abyssi
Fe 50-55 mol iron per mol protein Pyrodictium abyssi
Ni 1.6 mol per mol protein Pyrodictium abyssi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52000
-
 enzym complex, gel-filtration Pyrodictium abyssi

Organism

Organism UniProt Comment Textmining
Pyrodictium abyssi
-
 TAG 11, a sulfur-reducing complex
-

Oxidation Stability

Oxidation Stability Organism
the sulfur-reducing activity is irreversibly damaged by oxygen, anaerobic conditions: no loss of activity after one week, aerobic conditions: 80% loss of activity in 24 h Pyrodictium abyssi

Purification (Commentary)

Purification (Comment) Organism
enzyme complex Pyrodictium abyssi

Reaction

Reaction Comment Organism Reaction ID
H2 + (sulfide)n = hydrogen sulfide + (sulfide)n-1 a sulfur-reducing complex including hydrogenase, a sulfur reductase and electron-transferring components described Pyrodictium abyssi
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.56
-
 at 100°C Pyrodictium abyssi

Storage Stability

Storage Stability Organism
room temperature, exposed to air, 24 h, 80% loss of activity Pyrodictium abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfur + electron donor electron donor: molecular hydrogen Pyrodictium abyssi H2S + oxidized electron donor
-
 ?

Subunits

Subunits Comment Organism
More nine major polypeptides with molecular masses of 82000, 72000, 65000, 50000, 47000, 42000, 40000, 30000 and 24000 Da Pyrodictium abyssi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 enzyme extremely thermostable Pyrodictium abyssi
100
-
 below 85°C about 10% loss of activity Pyrodictium abyssi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Pyrodictium abyssi

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5
-
 Pyrodictium abyssi