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Literature summary for 1.12.98.4 extracted from

  • Schröder, I.; Kröger, A.; Macy, J.M.
    Isolation of the sulphur reductase and reconstittutions of the sulphur respiration of Wolinella succinogenes (1988), Arch. Microbiol., 149, 572-579.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information no lipophilic quinone, e.g. menaquinone (vitamin K2) involved, cytochrome b, c or covalently bound FAD are no cofactors Wolinella succinogenes

General Stability

General Stability Organism
anion exchange chromatography leads to severe loss of activity Wolinella succinogenes
gel filtration leads to severe loss of activity Wolinella succinogenes
sucrose density gradient centrifugation leads to severe loss of activity Wolinella succinogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Wolinella succinogenes 5737
-
membrane main activity membrane-bound Wolinella succinogenes 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe iron-sulfur (non-heme) protein Wolinella succinogenes
Fe 1 or 2 FeS-clusters per subunit Wolinella succinogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
85000
-
2 * 85000, SDS-PAGE Wolinella succinogenes
200000
-
calculated from sedimentation coefficient obtained by sucrose density gradient centrifugation and Stokes radius obtained from gel filtration Wolinella succinogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sulfur Wolinella succinogenes functional sulfur reductase that operates in the electron transport chain from fumarate to sulfur together with formate dehydrogenase, a membrane-bound and a cytoplasmic sulfur reductase H2S
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100 90% of the enzyme was solubilized Wolinella succinogenes

Organism

Organism UniProt Comment Textmining
Wolinella succinogenes
-
enzyme formed during growth at the expense of sulfur reduction by formate
-

Purification (Commentary)

Purification (Comment) Organism
isolation and incorporation into liposomes Wolinella succinogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
186
-
sulfide oxidation Wolinella succinogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sulfur functional sulfur reductase that operates in the electron transport chain from fumarate to sulfur together with formate dehydrogenase, a membrane-bound and a cytoplasmic sulfur reductase Wolinella succinogenes H2S
-
?
sulfur + electron donor electron donor: formate Wolinella succinogenes H2S + oxidized electron donor CO2 ?

Subunits

Subunits Comment Organism
dimer 2 * 85000, SDS-PAGE Wolinella succinogenes

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information liposomal preparation of membrane fraction: 2600 min-1, sulfide oxidation, liposomal preparation of enzyme: 1300 min-1, sulfide oxidation Wolinella succinogenes
additional information
-
additional information liposomal preparation of enzyme : 2000 min-1 Wolinella succinogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.1
-
sulfide oxidation Wolinella succinogenes