Literature summary for 1.12.98.4 extracted from
Schröder, I.; Kröger, A.; Macy, J.M.
Isolation of the sulphur reductase and reconstittutions of the sulphur respiration of Wolinella succinogenes (1988), Arch. Microbiol., 149, 572-579.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
additional information |
no lipophilic quinone, e.g. menaquinone (vitamin K2) involved, cytochrome b, c or covalently bound FAD are no cofactors |
Wolinella succinogenes |
|
General Stability
General Stability |
Organism |
anion exchange chromatography leads to severe loss of activity |
Wolinella succinogenes |
gel filtration leads to severe loss of activity |
Wolinella succinogenes |
sucrose density gradient centrifugation leads to severe loss of activity |
Wolinella succinogenes |
Localization
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
cytoplasm |
- |
Wolinella succinogenes |
5737 |
- |
membrane |
main activity membrane-bound |
Wolinella succinogenes |
16020 |
- |
Metals/Ions
Metals/Ions |
Comment |
Organism |
Structure |
Fe |
iron-sulfur (non-heme) protein |
Wolinella succinogenes |
|
Fe |
1 or 2 FeS-clusters per subunit |
Wolinella succinogenes |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
85000 |
- |
2 * 85000, SDS-PAGE |
Wolinella succinogenes |
200000 |
- |
calculated from sedimentation coefficient obtained by sucrose density gradient centrifugation and Stokes radius obtained from gel filtration |
Wolinella succinogenes |
Natural Substrates/ Products (Substrates)
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
sulfur |
Wolinella succinogenes |
functional sulfur reductase that operates in the electron transport chain from fumarate to sulfur together with formate dehydrogenase, a membrane-bound and a cytoplasmic sulfur reductase |
H2S |
- |
? |
|
Organic Solvent Stability
Organic Solvent |
Comment |
Organism |
Triton X-100 |
90% of the enzyme was solubilized |
Wolinella succinogenes |
Organism
Organism |
UniProt |
Comment |
Textmining |
Wolinella succinogenes |
- |
enzyme formed during growth at the expense of sulfur reduction by formate |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
isolation and incorporation into liposomes |
Wolinella succinogenes |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
186 |
- |
sulfide oxidation |
Wolinella succinogenes |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
sulfur |
functional sulfur reductase that operates in the electron transport chain from fumarate to sulfur together with formate dehydrogenase, a membrane-bound and a cytoplasmic sulfur reductase |
Wolinella succinogenes |
H2S |
- |
? |
|
sulfur + electron donor |
electron donor: formate |
Wolinella succinogenes |
H2S + oxidized electron donor |
CO2 |
? |
|
Subunits
Subunits |
Comment |
Organism |
dimer |
2 * 85000, SDS-PAGE |
Wolinella succinogenes |
Turnover Number [1/s]
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
additional information |
- |
additional information |
liposomal preparation of membrane fraction: 2600 min-1, sulfide oxidation, liposomal preparation of enzyme: 1300 min-1, sulfide oxidation |
Wolinella succinogenes |
|
additional information |
- |
additional information |
liposomal preparation of enzyme : 2000 min-1 |
Wolinella succinogenes |
|
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
8.1 |
- |
sulfide oxidation |
Wolinella succinogenes |