Cloned (Comment) | Organism |
---|---|
- |
Methanopyrus kandleri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cyanide | 1 mM, 50% inhibition | Methanothermobacter marburgensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
H2 | apparent Km for H2 is 60% | Methanothermobacter wolfeii | |
additional information | - |
H2 | the apparent Km for H2 is estimated to be approximately 60% H2 in the gas phase | Methanothermobacter marburgensis | |
0.02 | - |
5,10-methylenetetrahydromethanopterin | pH and temperature not specified in the publication | Methanothermobacter wolfeii | |
0.04 | - |
5,10-methylenetetrahydromethanopterin | pH 6.5, 60°C | Methanothermobacter marburgensis | |
0.05 | - |
5,10-methenyltetrahydromethanopterin | pH 7.5, 60°C | Methanothermobacter marburgensis | |
0.07 | - |
5,10-methenyltetrahydromethanopterin | pH and temperature not specified in the publication | Methanothermobacter wolfeii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme does not contain iron-sulfur clusters, nickel, or flavins | Methanothermobacter marburgensis | |
Zn2+ | the zinc content varies from preparation to preparation between 0.5-4 mol zinc/mol enzyme | Methanothermobacter marburgensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
- |
Methanothermobacter marburgensis |
43000 | - |
2 * 43000, SDS-PAGE | Methanothermobacter wolfeii |
92000 | - |
- |
Methanothermobacter wolfeii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | Q02394 | - |
- |
Methanopyrus kandleri DSM 6324 | Q02394 | - |
- |
Methanothermobacter marburgensis | P32440 | - |
- |
Methanothermobacter marburgensis DSM 2133 | P32440 | - |
- |
Methanothermobacter wolfeii | P32441 | - |
- |
Methanothermobacter wolfeii DSM 2970 | P32441 | - |
- |
Oxidation Stability | Organism |
---|---|
rapidly inactivated in the presence of molecular oxygen. The rate of inactivation increases in the presence of dithiothreitol (2 mM) or of mercaptoethanol (5 mM) | Methanothermobacter marburgensis |
the enzyme is much more sensitive towards inactivation by O2 than the enzyme from Methanothermobacter marburgensis | Methanothermobacter wolfeii |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter marburgensis |
- |
Methanothermobacter wolfeii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1250 | - |
pH 6, 60°C | Methanothermobacter marburgensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + 5,10-methylenetetrahydromethanopterin | - |
Methanothermobacter wolfeii | H2 + 5,10-methenyltetrahydromethanopterin | - |
r | |
H+ + 5,10-methylenetetrahydromethanopterin | the purified enzyme does not catalyze the reduction of viologen dyes, of methylene blue, of flavins, of pyridine nucleotides | Methanothermobacter marburgensis | H2 + 5,10-methenyltetrahydromethanopterin | - |
r | |
H+ + 5,10-methylenetetrahydromethanopterin | - |
Methanothermobacter wolfeii DSM 2970 | H2 + 5,10-methenyltetrahydromethanopterin | - |
r | |
H+ + 5,10-methylenetetrahydromethanopterin | the purified enzyme does not catalyze the reduction of viologen dyes, of methylene blue, of flavins, of pyridine nucleotides | Methanothermobacter marburgensis DSM 2133 | H2 + 5,10-methenyltetrahydromethanopterin | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 43000, SDS-PAGE | Methanothermobacter wolfeii |
homodimer | - |
Methanothermobacter marburgensis |
Synonyms | Comment | Organism |
---|---|---|
H2-forming methylenetetrahydromethanopterin dehydrogenase | - |
Methanothermobacter marburgensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
in 120 mM potassium phosphate pH 6 | Methanothermobacter marburgensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
30 min, 50% inactivation | Methanothermobacter marburgensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
975 | - |
5,10-methenyltetrahydromethanopterin | pH 7.5, 60°C | Methanothermobacter marburgensis | |
1462 | - |
5,10-methylenetetrahydromethanopterin | pH 6.5, 60°C | Methanothermobacter marburgensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Methanothermobacter marburgensis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Methanothermobacter marburgensis | isoelectric focussing | - |
4.4 |