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Literature summary for 1.12.98.2 extracted from

  • Zirngibl, C.; Van Dongen, W.; Schwörer, B.; Von Bünau, R.; Richter, M.; Klein, A.; Thauer, R.K.
    H2-forming methylenetetrahydromethanopterin dehydrogenase, a novel type of hydrogenase without iron-sulfur clusters in methanogenic archaea (1992), Eur. J. Biochem., 208, 511-520.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Methanopyrus kandleri

Inhibitors

Inhibitors Comment Organism Structure
cyanide 1 mM, 50% inhibition Methanothermobacter marburgensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
H2 apparent Km for H2 is 60% Methanothermobacter wolfeii
additional information
-
H2 the apparent Km for H2 is estimated to be approximately 60% H2 in the gas phase Methanothermobacter marburgensis
0.02
-
5,10-methylenetetrahydromethanopterin pH and temperature not specified in the publication Methanothermobacter wolfeii
0.04
-
5,10-methylenetetrahydromethanopterin pH 6.5, 60°C Methanothermobacter marburgensis
0.05
-
5,10-methenyltetrahydromethanopterin pH 7.5, 60°C Methanothermobacter marburgensis
0.07
-
5,10-methenyltetrahydromethanopterin pH and temperature not specified in the publication Methanothermobacter wolfeii

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme does not contain iron-sulfur clusters, nickel, or flavins Methanothermobacter marburgensis
Zn2+ the zinc content varies from preparation to preparation between 0.5-4 mol zinc/mol enzyme Methanothermobacter marburgensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
-
Methanothermobacter marburgensis
43000
-
2 * 43000, SDS-PAGE Methanothermobacter wolfeii
92000
-
-
Methanothermobacter wolfeii

Organism

Organism UniProt Comment Textmining
Methanopyrus kandleri Q02394
-
-
Methanopyrus kandleri DSM 6324 Q02394
-
-
Methanothermobacter marburgensis P32440
-
-
Methanothermobacter marburgensis DSM 2133 P32440
-
-
Methanothermobacter wolfeii P32441
-
-
Methanothermobacter wolfeii DSM 2970 P32441
-
-

Oxidation Stability

Oxidation Stability Organism
rapidly inactivated in the presence of molecular oxygen. The rate of inactivation increases in the presence of dithiothreitol (2 mM) or of mercaptoethanol (5 mM) Methanothermobacter marburgensis
the enzyme is much more sensitive towards inactivation by O2 than the enzyme from Methanothermobacter marburgensis Methanothermobacter wolfeii

Purification (Commentary)

Purification (Comment) Organism
-
Methanothermobacter marburgensis
-
Methanothermobacter wolfeii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1250
-
pH 6, 60°C Methanothermobacter marburgensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H+ + 5,10-methylenetetrahydromethanopterin
-
Methanothermobacter wolfeii H2 + 5,10-methenyltetrahydromethanopterin
-
r
H+ + 5,10-methylenetetrahydromethanopterin the purified enzyme does not catalyze the reduction of viologen dyes, of methylene blue, of flavins, of pyridine nucleotides Methanothermobacter marburgensis H2 + 5,10-methenyltetrahydromethanopterin
-
r
H+ + 5,10-methylenetetrahydromethanopterin
-
Methanothermobacter wolfeii DSM 2970 H2 + 5,10-methenyltetrahydromethanopterin
-
r
H+ + 5,10-methylenetetrahydromethanopterin the purified enzyme does not catalyze the reduction of viologen dyes, of methylene blue, of flavins, of pyridine nucleotides Methanothermobacter marburgensis DSM 2133 H2 + 5,10-methenyltetrahydromethanopterin
-
r

Subunits

Subunits Comment Organism
dimer 2 * 43000, SDS-PAGE Methanothermobacter wolfeii
homodimer
-
Methanothermobacter marburgensis

Synonyms

Synonyms Comment Organism
H2-forming methylenetetrahydromethanopterin dehydrogenase
-
Methanothermobacter marburgensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
in 120 mM potassium phosphate pH 6 Methanothermobacter marburgensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
30 min, 50% inactivation Methanothermobacter marburgensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
975
-
5,10-methenyltetrahydromethanopterin pH 7.5, 60°C Methanothermobacter marburgensis
1462
-
5,10-methylenetetrahydromethanopterin pH 6.5, 60°C Methanothermobacter marburgensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Methanothermobacter marburgensis

pI Value

Organism Comment pI Value Maximum pI Value
Methanothermobacter marburgensis isoelectric focussing
-
4.4