KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
H+ | pH 5.7 | Methanopyrus kandleri | |
0.05 | - |
N5,N10-methylenetetrahydromethanopterin | - |
Methanopyrus kandleri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | the enzyme requires the presence of salts, 50% of maximal activity is reached at 100 mM potassium phosphate, KCl or NaCl | Methanopyrus kandleri | |
NaCl | the enzyme requires the presence of salts, 50% of maximal activity is reached at 100 mM potassium phosphate, KCl or NaCl | Methanopyrus kandleri | |
potassium phosphate | the enzyme requires the presence of salts, 50% of maximal activity is reached at 100 mM potassium phosphate, KCl or NaCl | Methanopyrus kandleri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
4 * 44000, SDS-PAGE | Methanopyrus kandleri |
170000 | - |
gel filtration | Methanopyrus kandleri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methanopyrus kandleri | the enzyme is involved in methanogenesis from CO2 and H2 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanopyrus kandleri | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanopyrus kandleri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
360 | - |
- |
Methanopyrus kandleri |
Storage Stability | Organism |
---|---|
-20°C, under N2 gas phase, 20% loss of activity within 2 weeks | Methanopyrus kandleri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is involved in methanogenesis from CO2 and H2 | Methanopyrus kandleri | ? | - |
? | |
N5,N10-methylenetetrahydromethanopterin + H+ | r | Methanopyrus kandleri | N5,N10-methenyltetrahydromethanopterin + H2 | - |
? | |
N5,N10-methylenetetrahydromethanopterin + H+ | no reaction with: coenzyme F420, NAD+, NADP+ | Methanopyrus kandleri | N5,N10-methenyltetrahydromethanopterin + H2 | - |
? | |
N5,N10-methylenetetrahydromethanopterin + H+ | reverse reaction is favoured under alkaline conditions | Methanopyrus kandleri | N5,N10-methenyltetrahydromethanopterin + H2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 44000, SDS-PAGE | Methanopyrus kandleri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Methanopyrus kandleri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
60 min, stable, enzyme in cell extract | Methanopyrus kandleri |
90 | - |
rapid inactivation of purified enzyme | Methanopyrus kandleri |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | reaction rate increases hyperbolically with the proton concentration between pH 7 and pH 4.5 | Methanopyrus kandleri |