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Literature summary for 1.12.98.2 extracted from

  • Ma, M.; Zirngibl, C.; Linder, D.; Stetter, K.O.; Thauer, R.K.
    N5,N10-Methylenetetrahydromethanopterin dehydrogenase (H2-forming) from the extreme thermophile Methanopyrus kandleri (1991), Arch. Microbiol., 156, 43-48.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
H+ pH 5.7 Methanopyrus kandleri
0.05
-
N5,N10-methylenetetrahydromethanopterin
-
Methanopyrus kandleri

Metals/Ions

Metals/Ions Comment Organism Structure
KCl the enzyme requires the presence of salts, 50% of maximal activity is reached at 100 mM potassium phosphate, KCl or NaCl Methanopyrus kandleri
NaCl the enzyme requires the presence of salts, 50% of maximal activity is reached at 100 mM potassium phosphate, KCl or NaCl Methanopyrus kandleri
potassium phosphate the enzyme requires the presence of salts, 50% of maximal activity is reached at 100 mM potassium phosphate, KCl or NaCl Methanopyrus kandleri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
4 * 44000, SDS-PAGE Methanopyrus kandleri
170000
-
gel filtration Methanopyrus kandleri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Methanopyrus kandleri the enzyme is involved in methanogenesis from CO2 and H2 ?
-
?

Organism

Organism UniProt Comment Textmining
Methanopyrus kandleri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanopyrus kandleri

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
360
-
-
Methanopyrus kandleri

Storage Stability

Storage Stability Organism
-20°C, under N2 gas phase, 20% loss of activity within 2 weeks Methanopyrus kandleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is involved in methanogenesis from CO2 and H2 Methanopyrus kandleri ?
-
?
N5,N10-methylenetetrahydromethanopterin + H+ r Methanopyrus kandleri N5,N10-methenyltetrahydromethanopterin + H2
-
?
N5,N10-methylenetetrahydromethanopterin + H+ no reaction with: coenzyme F420, NAD+, NADP+ Methanopyrus kandleri N5,N10-methenyltetrahydromethanopterin + H2
-
?
N5,N10-methylenetetrahydromethanopterin + H+ reverse reaction is favoured under alkaline conditions Methanopyrus kandleri N5,N10-methenyltetrahydromethanopterin + H2
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 44000, SDS-PAGE Methanopyrus kandleri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
-
Methanopyrus kandleri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
60 min, stable, enzyme in cell extract Methanopyrus kandleri
90
-
rapid inactivation of purified enzyme Methanopyrus kandleri

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 reaction rate increases hyperbolically with the proton concentration between pH 7 and pH 4.5 Methanopyrus kandleri