Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.12.98.1 extracted from

  • Fox, J.A.; Livingston, D.J.; Orme-Johnson, W.H.; Walsh, C.T.
    8-Hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum: 1. Purification and characterization (1987), Biochemistry, 26, 4219-4227.
    View publication on PubMed
Search for more literature for 1.12.98.1

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-sulfur cluster Methanothermobacter thermautotrophicus
sulfur iron-sulfur cluster Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE Methanothermobacter thermautotrophicus
31000
-
 alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE Methanothermobacter thermautotrophicus
47000
-
 alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE Methanothermobacter thermautotrophicus
115000
-
 smallest species with enzymatic activity, native SDS-PAGE Methanothermobacter thermautotrophicus
800000
-
 gel filtration, native SDS-PAGE Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + coenzyme F420 Methanothermobacter thermautotrophicus enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
-
 strain delta H
-

Oxidation Stability

Oxidation Stability Organism
aerobic purification yields the stable but inactive enzyme, can be reactivated under reducing conditions Methanothermobacter thermautotrophicus

Purification (Commentary)

Purification (Comment) Organism
aerobic purification causes reversible inhibition with O2 Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
49
-
 purified enzyme, F0-linked activity Methanothermobacter thermautotrophicus

Storage Stability

Storage Stability Organism
-20°C, 1 year, inactive form maintains 70% of initial activity after reactivation Methanothermobacter thermautotrophicus
room temperature, activated enzyme loses 20% of inital activity within 24 h Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + coenzyme F0
-
 Methanothermobacter thermautotrophicus reduced coenzyme F0
-
 ?
H2 + coenzyme F420
-
 Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?
H2 + coenzyme F420 enzyme of methanogenesis pathway Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?

Subunits

Subunits Comment Organism
multimer alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE Methanothermobacter thermautotrophicus
multimer ratio alpha, beta, gamma postulated to be 1:1:1 Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5 F420 reduction Methanothermobacter thermautotrophicus
11
-
 optimum pH for methyl viologen reduction above pH 11 Methanothermobacter thermautotrophicus

pH Range

pH Minimum pH Maximum Comment Organism
5 9 F420 reduction Methanothermobacter thermautotrophicus
7 11 increasing methyl viologen activity Methanothermobacter thermautotrophicus

Cofactor

Cofactor Comment Organism Structure
FAD 0.8-0.9 mol per mol 115000 Da species, bound to gamma subunit Methanothermobacter thermautotrophicus