Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | iron-sulfur cluster | Methanothermobacter thermautotrophicus | |
sulfur | iron-sulfur cluster | Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE | Methanothermobacter thermautotrophicus |
31000 | - |
alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE | Methanothermobacter thermautotrophicus |
47000 | - |
alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE | Methanothermobacter thermautotrophicus |
115000 | - |
smallest species with enzymatic activity, native SDS-PAGE | Methanothermobacter thermautotrophicus |
800000 | - |
gel filtration, native SDS-PAGE | Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F420 | Methanothermobacter thermautotrophicus | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
strain delta H | - |
Oxidation Stability | Organism |
---|---|
aerobic purification yields the stable but inactive enzyme, can be reactivated under reducing conditions | Methanothermobacter thermautotrophicus |
Purification (Comment) | Organism |
---|---|
aerobic purification causes reversible inhibition with O2 | Methanothermobacter thermautotrophicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
49 | - |
purified enzyme, F0-linked activity | Methanothermobacter thermautotrophicus |
Storage Stability | Organism |
---|---|
-20°C, 1 year, inactive form maintains 70% of initial activity after reactivation | Methanothermobacter thermautotrophicus |
room temperature, activated enzyme loses 20% of inital activity within 24 h | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F0 | - |
Methanothermobacter thermautotrophicus | reduced coenzyme F0 | - |
? | |
H2 + coenzyme F420 | - |
Methanothermobacter thermautotrophicus | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanothermobacter thermautotrophicus | reduced coenzyme F420 | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | alpha,beta,gamma 8 * 47000 + 8 * 31000 + 8 * 26000, SDS-PAGE | Methanothermobacter thermautotrophicus |
multimer | ratio alpha, beta, gamma postulated to be 1:1:1 | Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | F420 reduction | Methanothermobacter thermautotrophicus |
11 | - |
optimum pH for methyl viologen reduction above pH 11 | Methanothermobacter thermautotrophicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | F420 reduction | Methanothermobacter thermautotrophicus |
7 | 11 | increasing methyl viologen activity | Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 0.8-0.9 mol per mol 115000 Da species, bound to gamma subunit | Methanothermobacter thermautotrophicus |