Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ammonium sulfate | improves reactivation of enzyme with FAD after FAD depletion | Methanobacterium formicicum | |
FAD | restores activity after depletion of FAD in hydrophobic interaction chromatography | Methanobacterium formicicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | - |
Methanobacterium formicicum | |
Nickel | - |
Methanobacterium formicicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
170000 | - |
gel filtration | Methanobacterium formicicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F420 | Methanobacterium formicicum | enzyme of methanogenesis pathway | reduced coenzyme F420 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanobacterium formicicum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanobacterium formicicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.24 | - |
F420-linked activity | Methanobacterium formicicum |
63.2 | - |
methyl viologen-linked activity | Methanobacterium formicicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + coenzyme F420 | - |
Methanobacterium formicicum | reduced coenzyme F420 | - |
? | |
H2 + coenzyme F420 | enzyme of methanogenesis pathway | Methanobacterium formicicum | reduced coenzyme F420 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | bound to protein, depleted by hydrophobic interaction chromatography | Methanobacterium formicicum | |
additional information | no activity with FMN after FAD depletion | Methanobacterium formicicum |