Literature summary for 1.12.7.2 extracted from

McTernan, P.M.; Chandrayan, S.K.; Wu, C.H.; Vaccaro, B.J.; Lancaster, W.A.; Adams, M.W.
Engineering the respiratory membrane-bound hydrogenase of the hyperthermophilic archaeon Pyrococcus furiosus and characterization of the catalytically active cytoplasmic subcomplex (2015), Protein Eng. Des. Sel., 28, 1-8.

Search for more literature for 1.12.7.2

Protein Variants

Protein Variants	Comment	Organism
additional information	engineering and expressing of a His-tagged minimal catalytically active 4-subunit cytoplasmic subcomplex of respiratory membrane-bound hydrogenase. In order to obtain a minimal subcomplex, the native MBH operon is splitt it into two separate transcriptional units	Pyrococcus furiosus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase	Pyrococcus furiosus	5737	-
membrane	native 14-subunit membrane-bound subcomplex of respiratory membrane-bound hydrogenase	Pyrococcus furiosus	16020	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe	contains [NiFe]-clusters. The Fe: Ni ratio of the engineered and expressed His-tagged 4-subunit subcomplex of respiratory membrane-bound hydrogenase. Subunit MbhL harbors the [NiFe] active site, subunit MbhK is part of the large subunit, subunit MbhJ harbors one [4Fe4S] cluster and MbhN harbors two [4Fe4S] clusters	Pyrococcus furiosus
Ni	contains [NiFe]-clusters. The Fe: Ni ratio of the engineered and expressed His-tagged 4-subunit subcomplex of respiratory membrane-bound hydrogenase. Subunit MbhL harbors the [NiFe] active site, subunit MbhK is part of the large subunit, subunit MbhJ harbors one [4Fe4S] cluster and MbhN harbors two [4Fe4S] clusters	Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
85000	-	gel filtration, engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase	Pyrococcus furiosus

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus furiosus	Q8U0Z8 and Q8U0Z7 and Q8U0Z6	Q8U0Z8: subunit mbhJ, Q8U0Z7: subunit mbhK, Q8U0Z6: catalytic subunit mbhL, subunit mbhN. Engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase, MbhJKLN	-

Oxidation Stability

Oxidation Stability	Organism
the O2 sensitivities of minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase and the native 14-subunit membrane-bound version are similar, half-lives of about 15 h in air	Pyrococcus furiosus

Purification (Commentary)

Purification (Comment)	Organism
purification of the engineered and expressed His-tagged 4-subunit cytoplasmic subcomplex of respiratory membrane-bound hydrogenase	Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + 2 oxidized ferredoxin	-	Pyrococcus furiosus	2 reduced ferredoxin + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
C-MBH	engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase	Pyrococcus furiosus
MbhJKLN	engineered minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase, MbhJKLN	Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	the minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase, H2 formation with the physiological electron donor, reduced ferredoxin	Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
90	-	half-life of minimal catalytically active 4-subunit subcomplex of respiratory membrane-bound hydrogenase: 8 h. Half-life of the native 14-subunit membrane-bound respiratory membrane-bound hydrogenase complex	Pyrococcus furiosus

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Release 2023.1 (January 2023)