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Literature summary for 1.12.7.2 extracted from

  • Ueno, Y.; Kurano, N.; Miyachi, S.
    Purification and characterization of hydrogenase from the marine green alga, Chlorococcum littorale (1999), FEBS Lett., 443, 144-148.
    View publication on PubMed
Search for more literature for 1.12.7.2

Inhibitors

Inhibitors Comment Organism Structure
O2 95% activity loss, few min at 4°C Alvikia littoralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00068
-
 oxidized ferredoxin
-
 Alvikia littoralis
0.5
-
 oxidized methyl viologen
-
 Alvikia littoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE Alvikia littoralis
57000
-
 gel filtration Alvikia littoralis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + oxidized ferredoxin Alvikia littoralis
-
 H+ + reduced ferredoxin
-
 r

Organism

Organism UniProt Comment Textmining
Alvikia littoralis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partially Alvikia littoralis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75.7
-
 hydrogen evolution, reduced methyl viologen as electron donor Alvikia littoralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + oxidized ferredoxin
-
 Alvikia littoralis H+ + reduced ferredoxin
-
 r
H2 + oxidized methyl viologen
-
 Alvikia littoralis H+ + reduced methyl viologen
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Alvikia littoralis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Alvikia littoralis