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Literature summary for 1.12.7.2 extracted from
- A novel type of iron hydrogenase in the green alga Scenedesmus obliquus is linked to the photosynthetic electron transport chain (2001), J. Biol. Chem., 276, 6125-6132.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Tetradesmus obliquus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone | inhibition of H2 production, photosystem 1 is involved in the supply of electrons to the hydrogenase | Tetradesmus obliquus |  |
| CO | strong inhibition | Tetradesmus obliquus | |
| O2 | strong inhibition | Tetradesmus obliquus | |
| sulfo-disalicylidinepropandiamine | hydrogenase activity is reduced up to 30-fold | Tetradesmus obliquus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
methyl viologen | - |
Tetradesmus obliquus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Tetradesmus obliquus | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | the catalytic site is an H-cluster, containing a [4Fe4S] subcluster and a [2Fe] center | Tetradesmus obliquus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
1 * 44000, SDS-PAGE | Tetradesmus obliquus |
| 44500 | - |
gel filtration and SDS-PAGE | Tetradesmus obliquus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized ferredoxin | Tetradesmus obliquus | ferredoxin links the enzyme to photosynthesis | H+ + reduced ferredoxin | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Tetradesmus obliquus | - |
wild-type Kützing 276-6 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| rapid inactivation under aerobic conditions | Tetradesmus obliquus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| strictly under anaerobic conditions | Tetradesmus obliquus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 700 | - |
hydrogen evolution | Tetradesmus obliquus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + electron acceptor | methyl viologen as electron acceptor | Tetradesmus obliquus | H+ + reduced electron acceptor | - |
r | |
| H2 + oxidized ferredoxin | ferredoxin links the enzyme to photosynthesis | Tetradesmus obliquus | H+ + reduced ferredoxin | - |
r | |
| H2 + oxidized methyl viologen | - |
Tetradesmus obliquus | H+ + reduced methyl viologen | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 44000, SDS-PAGE | Tetradesmus obliquus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Tetradesmus obliquus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Tetradesmus obliquus |
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Release 2023.1 (January 2023)
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