Application | Comment | Organism |
---|---|---|
industry | platinum (IV) recovery from an industrial effluent through a biosulphidogenic sulphate reducing bacteria consortium via a hydrogenase-cytochrome c3 enzyme system that removes electrons from hydrogen to the platinum metal that acts as a final electron acceptor | Bacteria |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 97% inhibition | Bacteria | |
Cr6+ | 1 mM, complete inhibition | Bacteria | |
Cu2+ | 1 mM, 95% inhibition | Bacteria | |
Zn2+ | 1 mM, 79% inhibition | Bacteria |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
methyl viologen | - |
Bacteria |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Bacteria | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | increases activity by 1.8% | Bacteria | |
Nickel | increases activity by 1.6% | Bacteria |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
x * 58000, SDS-PAGE | Bacteria |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteria | - |
sulphate reducing bacteria | - |
Purification (Comment) | Organism |
---|---|
by sonication, PEG 20000 concentration, ion exchange chromatography and gel filtration, 9.26fold purified with a yield of 51.8% | Bacteria |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3817 | - |
crude sonicate | Bacteria |
35340 | - |
9.26fold purified | Bacteria |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ferricytochrome c3 + 2 H2 | 48% relative activity compared to activity with oxidized methyl viologen | Bacteria | ferrocytochrome c3 + 4 H+ | - |
? | |
H2 + oxidized dichloroindophenol | 30% relative activity compared to activity with oxidized methyl viologen | Bacteria | H+ + ? | - |
? | |
H2 + oxidized methyl viologen | 100% relative activity | Bacteria | H+ + reduced methyl viologen | - |
? | |
H2 + potassium ferricyanide | 34% relative activity compared to activity with oxidized methyl viologen | Bacteria | H+ + ? | - |
? | |
additional information | hydrogen-dependent platinum (IV) reducing activity in the presence of hydrogenase and its physiological electron carrier, cytochrome c3 | Bacteria | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, SDS-PAGE | Bacteria |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | - |
Bacteria |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 40 | at optimal temperature range of 35-40°C, a minimal activity loss during the first 10 to 20 min and thereafter, activity steadily decreases as incubation time increases. After 80 min of incubation, the enzyme loses 75-80% of all of its activity | Bacteria |
55 | - |
completely denatured above | Bacteria |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
methyl viologen | - |
Bacteria |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Bacteria |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c3 | hydrogen-dependent platinum (IV) reducing activity in the presence of hydrogenase and its physiological electron carrier, cytochrome c3. Reduction of platinum by the purified hydrogenase enzyme alone yields about 10% removal after 3 h incubation while after the addition of cytochrome c3 there is a slight increase to 15%. Suppression of enzyme activity due to the extreme acid pH of 0.38 of the effluent | Bacteria |