Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga neapolitana |
Protein Variants | Comment | Organism |
---|---|---|
C302S | mutant displays a very weak electron paramagnetic resonance signal | Thermotoga neapolitana |
C353S | mutant displays a very weak electron paramagnetic resonance signal | Thermotoga neapolitana |
C356S | mutant displays a very weak electron paramagnetic resonance signal | Thermotoga neapolitana |
H304A | similar to wild-type, mutant exhibits electron paramagnetic resonance signals characteristic of oxidized [3Fe-4S]+ cluster | Thermotoga neapolitana |
H352A | similar to wild-type, mutant exhibits electron paramagnetic resonance signals characteristic of oxidized [3Fe-4S]+ cluster | Thermotoga neapolitana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga neapolitana | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
HydF | - |
Thermotoga neapolitana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4Fe-4S-center | displays electron paramagnetic resonance signals characteristic of oxidized [3Fe-4S]+ cluster. Cysteine residues C302, C353, and C356 are strictly required for the binding of the [4Fe-4S] cluster, whereas the fourth ligand of the coordination sphere can vary depending on the molecular environment created by local residues and/or experimental conditions | Thermotoga neapolitana |