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Literature summary for 1.12.1.4 extracted from

  • Verhagen, M.; Adams, M.
    Fe-only hydrogenase from Thermotoga maritima (2001), Methods Enzymol., 331, 216-226.
    View publication on PubMed

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the holoprotein contains 31.1 mol iron per mol enzyme Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18025
-
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence Thermotoga maritima
19000
-
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE Thermotoga maritima
68000
-
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE Thermotoga maritima
68676
-
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence Thermotoga maritima
72248
-
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence Thermotoga maritima
73000
-
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE Thermotoga maritima
158900
-
gel filtration Thermotoga maritima
160000
-
calculated from amino acid sequence Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NADH + H+ + reduced ferredoxin Thermotoga maritima
-
H2 + NAD+ + oxidized ferredoxin
-
?
NADH + H+ + reduced ferredoxin Thermotoga maritima DSM 3109
-
H2 + NAD+ + oxidized ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-
Thermotoga maritima DSM 3109
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NADH + H+ + reduced ferredoxin
-
Thermotoga maritima H2 + NAD+ + oxidized ferredoxin
-
?
NADH + H+ + reduced ferredoxin
-
Thermotoga maritima DSM 3109 H2 + NAD+ + oxidized ferredoxin
-
?
sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid
-
Thermotoga maritima H2 + oxidized anthraquinone-2,6-disulfonic acid + ?
-
?
sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid
-
Thermotoga maritima DSM 3109 H2 + oxidized anthraquinone-2,6-disulfonic acid + ?
-
?
sodium dithionite + H+ + reduced methyl viologen
-
Thermotoga maritima H2 + oxidized methyl viologen + ?
-
?
sodium dithionite + H+ + reduced methyl viologen
-
Thermotoga maritima DSM 3109 H2 + oxidized methyl viologen + ?
-
?

Subunits

Subunits Comment Organism
heterotrimer 1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence Thermotoga maritima
heterotrimer 1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE Thermotoga maritima

Synonyms

Synonyms Comment Organism
Fe-only hydrogenase
-
Thermotoga maritima

Cofactor

Cofactor Comment Organism Structure
flavin
-
Thermotoga maritima
iron-sulfur centre the alpha subunit contains two [2Fe-2S] clusters and three [4Fe-4S] clusters, the beta subunit contains three [4Fe-4S] clusters and one [2Fe-2S] clusters, the gamma subunit contains one [2Fe-2S] cluster Thermotoga maritima