Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the holoprotein contains 31.1 mol iron per mol enzyme | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18025 | - |
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence | Thermotoga maritima |
19000 | - |
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE | Thermotoga maritima |
68000 | - |
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE | Thermotoga maritima |
68676 | - |
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence | Thermotoga maritima |
72248 | - |
1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence | Thermotoga maritima |
73000 | - |
1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE | Thermotoga maritima |
158900 | - |
gel filtration | Thermotoga maritima |
160000 | - |
calculated from amino acid sequence | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + reduced ferredoxin | Thermotoga maritima | - |
H2 + NAD+ + oxidized ferredoxin | - |
? | |
NADH + H+ + reduced ferredoxin | Thermotoga maritima DSM 3109 | - |
H2 + NAD+ + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Thermotoga maritima DSM 3109 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Sepharose column chromatography, Q-Sepharose column chromatography, hydroxyapatite column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NADH + H+ + reduced ferredoxin | - |
Thermotoga maritima | H2 + NAD+ + oxidized ferredoxin | - |
? | |
NADH + H+ + reduced ferredoxin | - |
Thermotoga maritima DSM 3109 | H2 + NAD+ + oxidized ferredoxin | - |
? | |
sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid | - |
Thermotoga maritima | H2 + oxidized anthraquinone-2,6-disulfonic acid + ? | - |
? | |
sodium dithionite + H+ + reduced anthraquinone-2,6-disulfonic acid | - |
Thermotoga maritima DSM 3109 | H2 + oxidized anthraquinone-2,6-disulfonic acid + ? | - |
? | |
sodium dithionite + H+ + reduced methyl viologen | - |
Thermotoga maritima | H2 + oxidized methyl viologen + ? | - |
? | |
sodium dithionite + H+ + reduced methyl viologen | - |
Thermotoga maritima DSM 3109 | H2 + oxidized methyl viologen + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 72248 + 1 * 68676 + 1 * 18025, calculated from amino acid sequence | Thermotoga maritima |
heterotrimer | 1 * 73000 + 1 * 68000 + 1 * 19000, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
Fe-only hydrogenase | - |
Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Thermotoga maritima | |
iron-sulfur centre | the alpha subunit contains two [2Fe-2S] clusters and three [4Fe-4S] clusters, the beta subunit contains three [4Fe-4S] clusters and one [2Fe-2S] clusters, the gamma subunit contains one [2Fe-2S] cluster | Thermotoga maritima |