Cloned (Comment) | Organism |
---|---|
construction of a strain of Pyrococcus furiosus that contains a second set of the eight genes involved in the maturation of the catalytic subunit and insertion of the [NiFe]-site, along with a second set of the four genes encoding the NADPH-dependent cytoplasmic [NiFe]-hydrogenase (SHI) structural subunits. This results in a 40% higher yield of the purified affinity-tagged enzyme and the content of the inactive trimeric form decreases to 5% of the total protein | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + NADP+ | Pyrococcus furiosus | - |
H+ + NADPH | - |
? | |
H2 + NADP+ | Pyrococcus furiosus ATCC 43587 | - |
H+ + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | E7FHU4 AND E7FI44 | E7FHU4: sulfhydrogenase 1 subunit delta, E7FI44: sulfhydrogenase 1 subunit alpha | - |
Pyrococcus furiosus ATCC 43587 | E7FHU4 AND E7FI44 | E7FHU4: sulfhydrogenase 1 subunit delta, E7FI44: sulfhydrogenase 1 subunit alpha | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + NADP+ | - |
Pyrococcus furiosus | H+ + NADPH | - |
? | |
H2 + NADP+ | - |
Pyrococcus furiosus ATCC 43587 | H+ + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | - |
Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent [NiFe]-hydrogenase | - |
Pyrococcus furiosus |
SHI | - |
Pyrococcus furiosus |