Cloned (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | exclusively in | Thermococcus kodakarensis | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | NiFe-protein, the alphabetagammadelta subunit contains 0.8 mol of Ni | Thermococcus kodakarensis | |
Nickel | NiFe-protein, the alphabetagammadelta subunit contains 22.3 mol of Fe | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
440000 | - |
gel filtration | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | Q8NKS2 | alpha-subunit; KOD1 | - |
Thermococcus kodakarensis | Q8NKS3 | delta-subunit; KOD1 | - |
Thermococcus kodakarensis | Q8NKS4 | gamma-subunit; KOD1 | - |
Thermococcus kodakarensis | Q8NKS5 | beta-subunit; KOD1 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + 2,6-dichlorophenolindophenol | - |
Thermococcus kodakarensis | H+ + ? | - |
r | |
H2 + NAD+ | specific activity with NAD+ as electron acceptor is 150times lower than that of NADP+ | Thermococcus kodakarensis | H+ + NADH | - |
r | |
H2 + NADP+ | - |
Thermococcus kodakarensis | H+ + NADPH | - |
r | |
H2 + oxidized benzyl viologen | - |
Thermococcus kodakarensis | H+ + reduced benzyl viologen | - |
r | |
H2 + oxidized methyl viologen | - |
Thermococcus kodakarensis | H+ + reduced methyl viologen | - |
r | |
H2 + oxidized methylene blue | - |
Thermococcus kodakarensis | H+ + reduced methylene blue | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | (alphabetagammadelta)n, n is 3 or more | Thermococcus kodakarensis |
Synonyms | Comment | Organism |
---|---|---|
NiFe-hydrogenase | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
H2 production with methyl viologen | Thermococcus kodakarensis |
95 | - |
H2 uptake with methyl viologen | Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
203 min, 50% loss of activity of evolution reaction | Thermococcus kodakarensis |
90 | - |
18 min, 50% loss of activity of evolution reaction | Thermococcus kodakarensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the alphabetagammadelta subunit contains 1.01 mol of FAD | Thermococcus kodakarensis | |
NAD+ | specific activity as electron acceptor is 150times lower than that of NADP+ | Thermococcus kodakarensis | |
NADP+ | electron acceptor | Thermococcus kodakarensis |