Literature summary for 1.12.1.2 extracted from

van der Linden, E.; Burgdorf, T.; de Lacey, A.L.; Buhrke, T.; Scholte, M.; Fernandez, V.M.; Friedrich, B.; Albracht, S.P.
An improved purification procedure for the soluble [NiFe]-hydrogenase of Ralstonia eutropha: new insights into its (in)stability and spectroscopic properties (2006), J. Biol. Inorg. Chem., 11, 247-260.

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Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
soluble	-	Cupriavidus necator	-	-

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	-	-	-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1	-	-	-

Oxidation Stability

Oxidation Stability	Organism
air the presence of NADH leads to rapid destruction of the hexameric enzyme	Cupriavidus necator
the hexameric SH preparation is both active and stable in the presence of NADPH and air	Cupriavidus necator

Purification (Commentary)

Purification (Comment)	Organism
improved purification procedure	Cupriavidus necator

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	cells are grown heterotrophically in a fructose-glycerol minimal medium	Cupriavidus necator	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Cupriavidus necator

Synonyms

Synonyms	Comment	Organism
HoxFUYHI2	hexameric enzyme form	Cupriavidus necator
NAD+-reducing [NiFe]-hydrogenase	-	Cupriavidus necator

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Release 2023.1 (January 2023)