Organism | UniProt | Comment | Textmining |
---|---|---|---|
Coprinellus radians | - |
strain DSMZ 888 | - |
Cyclocybe aegerita | - |
strain TMA1 (DSMZ 22459) | - |
Purification (Comment) | Organism |
---|---|
ultrafiltration, ion exchange chromatography and gel filtration | Cyclocybe aegerita |
ultrafiltration, ion exchange chromatography and gel filtration | Coprinellus radians |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35 | - |
using veratryl alcohol as substrate, in 50 mM potassium phosphate buffer (pH 7.0), at 22°C over 15 min | Coprinellus radians |
62 | - |
using veratryl alcohol as substrate, in 50 mM potassium phosphate buffer (pH 7.0), at 22°C over 15 min | Cyclocybe aegerita |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-methylnaphthalene + H2O2 | - |
Cyclocybe aegerita | monohydroxylated 1-methylnaphthalene + dihydroxylated 1-methylnaphthalene + H2O | monohydroxylated 1-methylnaphthalene and dihydroxylated 1-methylnaphthalene are the main metabolites | ? | |
1-methylnaphthalene + H2O2 | - |
Coprinellus radians | monohydroxylated 1-methylnaphthalene + dihydroxylated 1-methylnaphthalene + H2O | monohydroxylated 1-methylnaphthalene and dihydroxylated 1-methylnaphthalene are the main metabolites | ? | |
1-pyrenol + H2O2 | - |
Cyclocybe aegerita | 1,8-dihydroxypyrene + 1,6-dihydroxypyrene + H2O | - |
? | |
1-pyrenol + H2O2 | - |
Coprinellus radians | 1,8-dihydroxypyrene + 1,6-dihydroxypyrene + H2O | - |
? | |
2-methylnaphthalene + H2O2 | - |
Cyclocybe aegerita | 2-naphthoic acid + monohydroxylated 2-methylnaphthalene + 2-naphthaldehyde + 2-naphthalene-methanol + monohydroxylated 2-naphthaldehyde + monohydroxylated 2-naphthoic acid + monohydroxylated 2-naphthalenemethanol + dihydroxylated 2-naphthalenemethanol + H2O | 2-naphthoic acid and monohydroxylated 2-methylnaphthalenes are the main metabolites | ? | |
2-methylnaphthalene + H2O2 | - |
Coprinellus radians | 2-naphthoic acid + monohydroxylated 2-methylnaphthalene + 2-naphthaldehyde + 2-naphthalene-methanol + monohydroxylated 2-naphthaldehyde + monohydroxylated 2-naphthoic acid + monohydroxylated 2-naphthalenemethanol + dihydroxylated 2-naphthalenemethanol + H2O | 2-naphthoic acid and monohydroxylated 2-methylnaphthalenes are the main metabolites | ? | |
anthracene + H2O2 | - |
Coprinellus radians | mono-hydroxylated anthracene + dihydroxylated anthracene | - |
? | |
anthracene + H2O2 | the enzyme almost completely converts anthracene within 6 h | Cyclocybe aegerita | mono-hydroxylated anthracene + dihydroxylated anthracene | - |
? | |
dibenzofuran + H2O2 | - |
Cyclocybe aegerita | 3-hydroxy-dibenzofuran + monohydroxylated dibenzofuran + 2,3-dihydroxydibenzofuran + 3,7-dihydroxydibenzofuran + dihydroxylated dibenzofuran + trihydroxylated dibenzofuran + H2O | - |
? | |
dibenzofuran + H2O2 | - |
Coprinellus radians | 3-hydroxy-dibenzofuran + monohydroxylated dibenzofuran + 2,3-dihydroxydibenzofuran + 3,7-dihydroxydibenzofuran + dihydroxylated dibenzofuran + trihydroxylated dibenzofuran + H2O | - |
? | |
fluorene + H2O2 | - |
Cyclocybe aegerita | 2-hydroxyfluorene + 9-fluorenol + dihydroxylated fluorene + monohydroxylated fluorenone + trihydroxylated fluorene + H2O | - |
? | |
fluorene + H2O2 | the enzyme oxygenates fluorene at the non-aromatic C9-carbon | Coprinellus radians | 9-fluorenone + 2-hydroxyfluorene + 9-fluorenol + dihydroxylated fluorene + monohydroxylated fluorenone + trihydroxylated fluorene + H2O | - |
? | |
additional information | the enzyme does not oxidize perylene | Cyclocybe aegerita | ? | - |
? | |
additional information | the enzyme does not oxidize phenanthrene and perylene | Coprinellus radians | ? | - |
? | |
phenanthrene + H2O2 | the enzyme almost completely converts phenantrene within 6 h | Cyclocybe aegerita | 4-phenanthrol + 1-phenanthrol + 3-phenanthrol + dihydroxylated phenanthrol + H2O | - |
? | |
pyrene + H2O2 | 13% of pyrene is oxidized within 8 h | Coprinellus radians | 1-pyrenol + H2O | - |
? | |
pyrene + H2O2 | 45% of pyrene is oxidized within 8 h | Cyclocybe aegerita | 1-pyrenol + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Cyclocybe aegerita | veratraldehyde + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | - |
Coprinellus radians | veratraldehyde + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APO | - |
Cyclocybe aegerita |
APO | - |
Coprinellus radians |
aromatic peroxygenase | - |
Cyclocybe aegerita |
aromatic peroxygenase | - |
Coprinellus radians |