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Literature summary for 1.11.2.1 extracted from

  • Anh, D.H.; Ullrich, R.; Benndorf, D.; Svatos, A.; Muck, A.; Hofrichter, M.
    The coprophilous mushroom Coprinus radians secretes a haloperoxidase that catalyzes aromatic peroxygenation (2007), Appl. Environ. Microbiol., 73, 5477-5485.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) in sodium phosphate-citrate buffer at pH 4.5 and 25°C Coprinellus radians
0.088
-
veratryl alcohol in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
0.342
-
2,6-dimethoxyphenol in sodium phosphate-citrate buffer at pH 4.5 and 25°C Coprinellus radians
0.584
-
Naphthalene in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
0.635
-
benzyl alcohol in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
1.201
-
H2O2 in the presence of 5 mM benzyl alcohol, in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Coprinellus radians
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
enzyme fractions P II and P III, SDS-PAGE Coprinellus radians
45000
-
enzyme fraction P I, SDS-PAGE Coprinellus radians

Organism

Organism UniProt Comment Textmining
Coprinellus radians
-
strain DSMZ 888
-

Oxidation Stability

Oxidation Stability Organism
an H2O2 concentration of 0.7 mM is most suitable regarding enzyme activity and stability Coprinellus radians

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the deglycosylated protein has a molecular mass of 27000 Da, indicating a high carbohydrate content of 37% of the mature protein Coprinellus radians

Purification (Commentary)

Purification (Comment) Organism
ultrafiltration, Q Sepharose column chromatography, Mono Q column chromatography, and SEC column chromatography Coprinellus radians

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.83
-
culture liquid, using veratryl alcohol as substrate, at pH 7.0 and 25°C Coprinellus radians
30.6
-
36.7fold purified enzyme fraction P II, using veratryl alcohol as substrate, at pH 7.0 and 25°C Coprinellus radians
31.5
-
37.8fold purified enzyme fraction P I, using veratryl alcohol as substrate, at pH 7.0 and 25°C Coprinellus radians
38.5
-
46.2fold purified enzyme fraction P III, using veratryl alcohol as substrate, at pH 7.0 and 25°C Coprinellus radians

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O2
-
Coprinellus radians ?
-
?
2,6-dimethoxyphenol + H2O2
-
Coprinellus radians ?
-
?
benzyl alcohol + H2O2
-
Coprinellus radians benzaldehyde + H2O
-
?
additional information the enzyme also brominates phenol to 2- and 4-bromophenols and selectively hydroxylates naphthalene to 1-naphthol, but shows no laccase activity Coprinellus radians ?
-
?
naphthalene + H2O2
-
Coprinellus radians 1-naphthol + H2O
-
?
veratryl alcohol + H2O2 + H+
-
Coprinellus radians veratraldehyde + H2O
-
?

Synonyms

Synonyms Comment Organism
haloperoxidase-peroxygenase
-
Coprinellus radians

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2
-
2,6-dimethoxyphenol in sodium phosphate-citrate buffer at pH 4.5 and 25°C Coprinellus radians
15
-
Naphthalene in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
34
-
veratryl alcohol in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
123
-
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) in sodium phosphate-citrate buffer at pH 4.5 and 25°C Coprinellus radians
176
-
benzyl alcohol in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
471
-
H2O2 in the presence of 5 mM benzyl alcohol, in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
pH optimum for the oxidation of 2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate) Coprinellus radians
6
-
pH optimum for the oxidation of 2,6-dimethoxyphenol Coprinellus radians
7
-
the optimum pH of the major fraction (P II) for the oxidation of aryl alcohols is around 7.0 Coprinellus radians
7.5
-
pH optimum for the oxidation of veratryl alcohol Coprinellus radians
8
-
pH optimum for the oxidation of benzyl alcohol Coprinellus radians

pH Range

pH Minimum pH Maximum Comment Organism
2.5 9 oxidation of veratryl alcohol and benzyl alcohol occurs in a broad pH range between 2.5 and 9.0 Coprinellus radians

Cofactor

Cofactor Comment Organism Structure
heme heme-thiolate protein Coprinellus radians

pI Value

Organism Comment pI Value Maximum pI Value
Coprinellus radians enzyme fraction P I shows two pI-values at 3.8 and 3.9, isoelectric focusing
-
3.8
Coprinellus radians enzyme fraction P I shows two pI-values at 3.8 and 3.9, isoelectric focusing
-
3.9
Coprinellus radians enzyme fraction P III, isoelectric focusing
-
4
Coprinellus radians enzyme fraction P II, isoelectric focusing
-
4.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.85
-
2,6-dimethoxyphenol in sodium phosphate-citrate buffer at pH 4.5 and 25°C Coprinellus radians
25.7
-
Naphthalene in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
277
-
benzyl alcohol in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
386
-
veratryl alcohol in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
392
-
H2O2 in the presence of 5 mM benzyl alcohol, in sodium phosphate-citrate buffer at pH 7.0 and 25°C Coprinellus radians
2510
-
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) in sodium phosphate-citrate buffer at pH 4.5 and 25°C Coprinellus radians