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Literature summary for 1.11.1.7 extracted from

  • Dalal, S.; Gupta, M.N.
    Purification and characterization of a peroxidase isozyme from Indian turnip roots (2010), J. Agric. Food Chem., 58, 5545-5552.
    View publication on PubMed
Search for more literature for 1.11.1.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 3,3',5,5'-tetramethylbenzidine native enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
0.045
-
 3,3',5,5'-tetramethylbenzidine pyromellitic dianhydride-modified enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
0.14
-
 guaiacol native enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
0.14
-
 guaiacol pyromellitic dianhydride-modified enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
0.18
-
 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) pyromellitic dianhydride-modified enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
0.19
-
 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) native enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
1.1
-
 4-Chlorophenol pyromellitic dianhydride-modified enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
1.12
-
 phenol pyromellitic dianhydride-modified enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
1.17
-
 phenol native enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
1.5
-
 4-Chlorophenol native enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
9.1
-
 o-Cresol pyromellitic dianhydride-modified enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
16.7
-
 o-Cresol native enzyme, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE Brassica rapa

Organism

Organism UniProt Comment Textmining
Brassica rapa
-
 Indian turnip
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Con A-Sepharose column chromatography, and DEAE-cellulose column chromatography Brassica rapa

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Brassica rapa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
125
-
 crude extract, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa
3441
-
 after 28fold purification, in 0.01 M phosphate buffer at pH 7.5 and 25°C Brassica rapa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
 Brassica rapa ?
-
 ?
3,3',5,5'-tetramethylbenzidine + H2O2
-
 Brassica rapa ?
-
 ?
4-chlorophenol + H2O2 in the absence of polyethylene glycol, pyromellitic dianhydride-modified peroxidase converts 100% 4-chlorophenol, while at the same time, native enzyme can convert only 85%. In the presence of polyethylene glycol, pyromellitic dianhydride-modified peroxidase converts 4-chlorophenol completely in 45 min, while native TP I requires 60 min for complete conversion Brassica rapa ?
-
 ?
guaiacol + H2O2
-
 Brassica rapa tetraguaiacol + H2O
-
 ?
o-cresol + H2O2
-
 Brassica rapa ?
-
 ?
phenol + H2O2
-
 Brassica rapa ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Brassica rapa

Synonyms

Synonyms Comment Organism
TP I
-
 Brassica rapa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 80 the pyromellitic dianhydride-modified enzyme retains 100% activity at 40°C after 15 min, while the native enzyme loses 15% activity. At 80°C, the native enzyme loses 95% activity, while at the same time, the pyromellitic dianhydride-modified enzyme is able to retain 25% activity. The native enzyme retains 75% activity at 50°C for 15 min Brassica rapa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 native enzyme Brassica rapa

Cofactor

Cofactor Comment Organism Structure
heme
-
 Brassica rapa