General Stability | Organism |
---|---|
cofactor modification(heminD1 in rHRP1 and heminD2 in rHRP2 instead of heme) increases the substrate affinity and catalytic efficiency both in aqueous buffer and some organic solvents, the catalytic efficiency for phenol oxidation is increased by about 55% for rHRP1 in aqueous buffer, and it is also increased by about 70% for rHRP1 in 10% (v/v) acetonitrile. | Armoracia rusticana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetonitrile | about 98% activity is lost for native HRP after incubation in 50% (v/v) acetonitrile at 35°C for 3 h, native HRP only possess less than 20% activity in 30% (v/v) acetonitrile | Armoracia rusticana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.36 | - |
4-Aminophenol | native enzyme, at 30°C | Armoracia rusticana | |
3.59 | - |
phenol | native enzyme, at 30°C | Armoracia rusticana | |
18.76 | - |
p-hydroxybenzoic acid | native enzyme, at 30°C | Armoracia rusticana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Armoracia rusticana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-aminoantipyrin + H2O2 | - |
Armoracia rusticana | ? | - |
? | |
4-aminophenol + H2O2 | - |
Armoracia rusticana | ? | - |
? | |
p-hydroxybenzoic acid + H2O2 | - |
Armoracia rusticana | ? | - |
? | |
phenol + H2O2 | - |
Armoracia rusticana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
horseradish peroxidase C | - |
Armoracia rusticana |
HRP | - |
Armoracia rusticana |
rHRP1 | apo-horseradish peroxidase constituted with the artificial prostethic group heminD1 | Armoracia rusticana |
rHRP2 | apo-horseradish peroxidase constituted with the artificial prostethic group heminD2 | Armoracia rusticana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 76.5 | the melting temperature is at 70°C for the native enzyme and at 75.4°C for the cofactor-modified enzyme rHRP1 and at 76.5°C for the cofactor-modified enzyme rHRP2. the reconstituted HRPs with modified hemin show higher thermostability in aqueous buffer. After the exposure for 1.5 h at 65°C, native HRP retains only about 15% activity, the reconstituted HRPs, however, retained about 60% activity | Armoracia rusticana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
311.7 | - |
p-hydroxybenzoic acid | native enzyme, at 30°C | Armoracia rusticana | |
691.7 | - |
phenol | native enzyme, at 30°C | Armoracia rusticana | |
1272 | - |
4-Aminophenol | native enzyme, at 30°C | Armoracia rusticana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Armoracia rusticana |