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Literature summary for 1.11.1.7 extracted from

  • Rudrappa, T.; Lakshmanan, V.; Kaunain, R.; Singara, N.M.; Neelwarne, B.
    Purification and characterization of an intracellular peroxidase from genetically transformed roots of red beet (Beta vulgaris L.) (2007), Food Chem., 105, 1312-1320.
    View publication on PubMed
Search for more literature for 1.11.1.7

Inhibitors

Inhibitors Comment Organism Structure
Periodate completely and competitively inhibited by periodate Beta vulgaris
Sodium azide non-competitive inhibitor Beta vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.113
-
 H2O2 at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris
2.134
-
 o-Dianisidine at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris
5.05
-
 fructosyl-6-phosphate alpha-Boc-L-lysine at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris
98.61
-
 guaiacol at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE Beta vulgaris

Organism

Organism UniProt Comment Textmining
Beta vulgaris
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation and DEAE cellulose column chromatography Beta vulgaris

Source Tissue

Source Tissue Comment Organism Textmining
root hair
-
 Beta vulgaris
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the enzyme from crude extract has a specific activity of 700 units the purified enzyme has a specific activity of 10500 units, one unit of enzyme activity refers to the rate of change of 1 OD per min per mg protein Beta vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Beta vulgaris ?
-
 ?
guaiacol + H2O2
-
 Beta vulgaris tetraguaiacol + H2O
-
 ?
o-dianisidine + H2O2
-
 Beta vulgaris ?
-
 ?

Synonyms

Synonyms Comment Organism
POD
-
 Beta vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 retains over 70% of the activity for 20 min at 70°C Beta vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Beta vulgaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 stable over a wide range at pH 3-9 with highest stability between pH 6 and 8 Beta vulgaris

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 Periodate at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) Beta vulgaris