KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
H2O2 | isoenzyme I | Cytobacillus firmus | |
36 | - |
H2O2 | isoenzyme II | Cytobacillus firmus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
x * 84000, isoenzyme I, x * 60000, isoenzyme II, x * 80000, isoenzyme III, SDS-PAGE | Cytobacillus firmus |
80000 | - |
x * 84000, isoenzyme I, x * 60000, isoenzyme II, x * 80000, isoenzyme III, SDS-PAGE | Cytobacillus firmus |
84000 | - |
x * 84000, isoenzyme I, x * 60000, isoenzyme II, x * 80000, isoenzyme III, SDS-PAGE | Cytobacillus firmus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | Cytobacillus firmus | two monofunctional, one bifunctional catalase-peroxidase | O2 + H2O | - |
? | |
H2O2 | Cytobacillus firmus OF4 | two monofunctional, one bifunctional catalase-peroxidase | O2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cytobacillus firmus | - |
- |
- |
Cytobacillus firmus OF4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
near homogeneity, 3 isoenzymes | Cytobacillus firmus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Cytobacillus firmus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4000 | - |
isoenzyme I | Cytobacillus firmus |
8000 | - |
isoenzyme II | Cytobacillus firmus |
75000 | - |
isoenzyme II | Cytobacillus firmus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | only isoenzyme I has peroxidase activity | Cytobacillus firmus | ? + H2O | - |
? | |
2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | only isoenzyme I has peroxidase activity | Cytobacillus firmus OF4 | ? + H2O | - |
? | |
H2O2 | two monofunctional, one bifunctional catalase-peroxidase | Cytobacillus firmus | O2 + H2O | - |
? | |
H2O2 | two monofunctional, one bifunctional catalase-peroxidase | Cytobacillus firmus OF4 | O2 + H2O | - |
? | |
o-methoxyphenol + H2O2 | only isoenzyme I has peroxidase activity | Cytobacillus firmus | ? + H2O | - |
? | |
o-methoxyphenol + H2O2 | i.e. guaiacol | Cytobacillus firmus | ? + H2O | - |
? | |
o-methoxyphenol + H2O2 | only isoenzyme I has peroxidase activity | Cytobacillus firmus OF4 | ? + H2O | - |
? | |
o-methoxyphenol + H2O2 | i.e. guaiacol | Cytobacillus firmus OF4 | ? + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 84000, isoenzyme I, x * 60000, isoenzyme II, x * 80000, isoenzyme III, SDS-PAGE | Cytobacillus firmus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
optimal activity below pH 6.5 | Cytobacillus firmus |
8 | - |
isoenzyme I | Cytobacillus firmus |
8 | 10.5 | isoenzyme II | Cytobacillus firmus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | isoenzymes I and II contain protoheme, isoenzyme II contains chlorine-type heme | Cytobacillus firmus |