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Literature summary for 1.11.1.6 extracted from
- Purification and characterization of a mesohalic catalase from the halophilic bacterium Halobacterium halobium (1995), J. Bacteriol., 177, 378-384.
General Stability
| General Stability | Organism |
|---|---|
| protein must be purified in the presence of 2 M NaCl, although its highest activity is observed in the absence of salt | Halobacterium salinarum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-amino-1H-1,2,4-triazole | 33% inactivation at 20 mM in 2.5 h | Halobacterium salinarum |  |
| CaCl2 | irreversible loss of activity at 2 M | Halobacterium salinarum | |
| KCN | - |
Halobacterium salinarum | |
| MgCl2 | irreversible loss of activity at 2 M | Halobacterium salinarum | |
| NaN3 | - |
Halobacterium salinarum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60 | - |
H2O2 | - |
Halobacterium salinarum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
4 * 62000, SDS-PAGE | Halobacterium salinarum |
| 240000 | - |
gel filtration | Halobacterium salinarum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 | Halobacterium salinarum | monofunctional catalase | O2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halobacterium salinarum | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| not sensitive to oxidation by singlet oxygen | Halobacterium salinarum |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Halobacterium salinarum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Halobacterium salinarum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | inducible at low salt concentrations | Halobacterium salinarum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6422 | - |
- |
Halobacterium salinarum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 2 M NaCl, 1 month | Halobacterium salinarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2O2 | monofunctional catalase | Halobacterium salinarum | O2 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 62000, SDS-PAGE | Halobacterium salinarum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
stable up to | Halobacterium salinarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Halobacterium salinarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 10 | high activity within | Halobacterium salinarum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | 0.78 protoheme per tetramer | Halobacterium salinarum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.002 | - |
NaN3 | - |
Halobacterium salinarum | |
| 0.018 | - |
KCN | - |
Halobacterium salinarum | |
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