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Literature summary for 1.11.1.5 extracted from

  • Pearl, N.M.; Jacobson, T.; Arisa, M.; Vitello, L.B.; Erman, J.E.
    Effect of single-site charge-reversal mutations on the catalytic properties of yeast cytochrome c peroxidase: mutations near the high-affinity cytochrome c binding site (2007), Biochemistry, 46, 8263-8272.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
D18K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
D210K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
D33K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
D34K the mutation causes large increases in the Michaelis constant indicating a reduced affinity for cytochrome c Saccharomyces cerevisiae
D37K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
E118K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
E17K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
E201K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
E209K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
E290K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
E291K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
E32K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
E35K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
E98K positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
K149D positive-to-negative charge-reversal mutant Saccharomyces cerevisiae
R31E positive-to-negative charge-reversal mutant Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 iso-1 ferrocytochrome c mutant enzyme D210K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.002
-
 iso-1 ferrocytochrome c mutant enzyme D18K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.0021
-
 iso-1 ferrocytochrome c recombinant wild type enzyme, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.0023
-
 iso-1 ferrocytochrome c mutant enzyme E17K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.0024
-
 iso-1 ferrocytochrome c mutant enzyme D33K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.0028
-
 iso-1 ferrocytochrome c mutant enzyme E209K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.0029
-
 iso-1 ferrocytochrome c mutant enzyme E201K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.0031
-
 iso-1 ferrocytochrome c mutant enzyme E98K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.0035
-
 iso-1 ferrocytochrome c mutant enzyme E35K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.0038
-
 iso-1 ferrocytochrome c mutant enzyme E291K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.0045
-
 iso-1 ferrocytochrome c mutant enzyme E32K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.051
-
 iso-1 ferrocytochrome c mutant enzyme E118K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.06
-
 iso-1 ferrocytochrome c mutant enzyme E290K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.082
-
 iso-1 ferrocytochrome c mutant enzyme D37K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.1
-
 iso-1 ferrocytochrome c Km above 0.1 mM, mutant enzyme D34K, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae
0.1
-
 iso-1 ferrocytochrome c Km above 0.1 mM, mutant enzyme R31E, in 0.1 M potassium phosphate buffer, pH 7.5, at 25°C Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
iso-1 ferrocytochrome c + H2O2
-
 Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
CCP
-
 Saccharomyces cerevisiae