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Literature summary for 1.11.1.5 extracted from

  • Guo, M.; Bhaskar, B.; Li, H.; Barrows, T.P.; Poulos, T.L.
    Crystal structure and characterization of a cytochrome c peroxidase-cytochrome c site-specific cross-link (2004), Proc. Natl. Acad. Sci. USA, 101, 5940-5945.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of V197C/C128A mutant in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
with comercial kit Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
V197C/C128A as active as the wild-type enzyme. Used to generate a covalent complex with a mutant cytochrome c Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ferrocytochrome c + H2O2 Saccharomyces cerevisiae
-
ferricytochrome c + H2O
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
high-purity V197C/C128A mutant is obtained after an anion-exchange chromatography and gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferrocytochrome c + H2O2
-
Saccharomyces cerevisiae ferricytochrome c + H2O
-
?
horse heart ferrocytochrome c + H2O2
-
Saccharomyces cerevisiae horse heart ferricytochrome c + H2O
-
r
yeast ferrocytochrome c + H2O2
-
Saccharomyces cerevisiae yeast ferricytochrome c + H2O
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
horse heart ferrocytochrome c pH 6, 200 mM potassium phosphate, covalent complex Saccharomyces cerevisiae
15.7
-
yeast ferrocytochrome c pH 6, 20 mM potassium phosphate, covalent complex Saccharomyces cerevisiae
20.7
-
horse heart ferrocytochrome c pH 6, 20 mM potassium phosphate, covalent complex Saccharomyces cerevisiae
76.2
-
yeast ferrocytochrome c pH 6, 200 mM potassium phosphate, covalent complex Saccharomyces cerevisiae
166.8
-
horse heart ferrocytochrome c pH 6, 200 mM potassium phosphate, V197C/C128A mutant Saccharomyces cerevisiae
175.1
-
horse heart ferrocytochrome c pH 6, 200 mM potassium phosphate, wild-type enzyme Saccharomyces cerevisiae
219.1
-
yeast ferrocytochrome c pH 6, 20 mM potassium phosphate, wild-type enzyme Saccharomyces cerevisiae
240.9
-
yeast ferrocytochrome c pH 6, 20 mM potassium phosphate, V197C/C128A mutant Saccharomyces cerevisiae
803.4
-
horse heart ferrocytochrome c pH 6, 20 mM potassium phosphate, V197C/C128A mutant Saccharomyces cerevisiae
850.3
-
horse heart ferrocytochrome c pH 6, 20 mM potassium phosphate, wild-type enzyme Saccharomyces cerevisiae
1167
-
yeast ferrocytochrome c pH 6, 200 mM potassium phosphate, V197C/C128A mutant Saccharomyces cerevisiae
1362
-
yeast ferrocytochrome c pH 6, 200 mM potassium phosphate, wild-type enzyme Saccharomyces cerevisiae