Literature summary for 1.11.1.21 extracted from

Philibert, T.; Rao, Z.; Yang, T.; Zhou, J.; Huang, G.; Irene, K.; Samuel, N.
Heterologous expression and characterization of a new heme-catalase in Bacillus subtilis 168 (2016), J. Ind. Microbiol. Biotechnol., 43, 729-740 .

Search for more literature for 1.11.1.21

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Bacillus subtilis	Bacillus pumilus

Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular modeling of structure reveals highly conserved catalytic residues His57 and Asn130 in the heme cavity and Asp110 in the main channel	Bacillus pumilus

General Stability

General Stability	Organism
20°C, 20 h, 90% residual activity	Bacillus pumilus

Inhibitors

Inhibitors	Comment	Organism	Structure
3-Amino-1,2,4-triazole	5 mM, 35% inhibition	Bacillus pumilus
3-Amino-4-hydroxybenzoic acid	50 mM, 30% residual activity	Bacillus pumilus
Co2+	1 mM, 25% residual activity	Bacillus pumilus
Cu2+	1 mM, 30% residual activity	Bacillus pumilus
hydroxylammonium chloride	0.5 mM, 70% inhibition	Bacillus pumilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.97	-	peroxyacetic acid	pH 4.5, 37°C	Bacillus pumilus
59.6	-	H2O2	pH 7.0, 37°C	Bacillus pumilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	1 mM, 110% of initial activity	Bacillus pumilus

Organism

Organism	UniProt	Comment	Textmining
Bacillus pumilus	A0A143G976	-	-
Bacillus pumilus ML 413	A0A143G976	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
55784	-	substrate H2O2, pH 7.0, 37°C	Bacillus pumilus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 H2O2	-	Bacillus pumilus	2 H2O + O2	-	?
2 H2O2	-	Bacillus pumilus ML 413	2 H2O + O2	-	?
peroxyacetic acid + 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)	-	Bacillus pumilus	?	-	?
peroxyacetic acid + 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid)	-	Bacillus pumilus ML 413	?	-	?

Synonyms

Synonyms	Comment	Organism
katX2	-	Bacillus pumilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Bacillus pumilus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	rapid decrease in stability	Bacillus pumilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
19.42	-	peroxyacetic acid	pH 4.5, 37°C	Bacillus pumilus
322000	-	H2O2	pH 7.0, 37°C	Bacillus pumilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Bacillus pumilus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	11	-	Bacillus pumilus
9	-	14 h, 80% residual activity	Bacillus pumilus

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Bacillus pumilus

General Information

General Information	Comment	Organism
physiological function	expression in Bacillus subtilis improves its resistance to oxidative stress	Bacillus pumilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
20.02	-	peroxyacetic acid	pH 4.5, 37°C	Bacillus pumilus
5410	-	H2O2	pH 7.0, 37°C	Bacillus pumilus

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Release 2023.1 (January 2023)