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Literature summary for 1.11.1.21 extracted from

  • Deemagarn, T.; Wiseman, B.; Carpena, X.; Ivancich, A.; Fita, I.; Loewen, P.C.
    Two alternative substrate paths for compound I formation and reduction in catalase-peroxidase KatG from Burkholderia pseudomallei (2007), Proteins Struct. Funct. Bioinform., 66, 219-228.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme D141E Burkholderia pseudomallei

Protein Variants

Protein Variants Comment Organism
D141A the mutant shows decreased catalase and peroxidase activities Burkholderia pseudomallei
D141E the mutant retains normal catalase activity and decreased peroxidase activity Burkholderia pseudomallei
D141N the mutant shows decreased catalase and peroxidase activities Burkholderia pseudomallei
R108A the mutation causes a reduction in catalase activity to 35% of native levels and a decrease in peroxidase activity Burkholderia pseudomallei
R108A/D141A the mutant exhibits near normal catalase activity (82% of native) and decreased peroxidase activity Burkholderia pseudomallei
R108A/W111F the mutant shows stronglydecreased catalase and peroxidase activities Burkholderia pseudomallei
R108A/W111F/D141A the mutant shows strongly decreased catalase and peroxidase activities Burkholderia pseudomallei
R108K the mutant shows decreased catalase and peroxidase activities Burkholderia pseudomallei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0055
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.0092
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.024
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.03
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.07
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.07
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.09
-
H2O2 mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.12
-
H2O2 mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.14
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) wild type enzyme, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.19
-
H2O2 mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.27
-
H2O2 mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.31
-
H2O2 wild type enzyme, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.41
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.5
-
H2O2 mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.55
-
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.56
-
H2O2 mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.92
-
H2O2 mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.98
-
H2O2 mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
3.7
-
H2O2 wild type enzyme, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
13
-
H2O2 mutant enzyme R108A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
32
-
H2O2 mutant enzyme R108A/D141A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
35
-
H2O2 mutant enzyme R108A/W111F/D141A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
36
-
H2O2 mutant enzyme R108A/W111F, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
60
-
H2O2 mutant enzyme D141A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
75
-
H2O2 mutant enzyme D141E, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
95
-
H2O2 mutant enzyme D141N, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
180
-
H2O2 mutant enzyme R108K, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei

Organism

Organism UniProt Comment Textmining
Burkholderia pseudomallei Q939D2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Burkholderia pseudomallei oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
H2O2
-
Burkholderia pseudomallei O2 + H2O
-
?

Synonyms

Synonyms Comment Organism
KatG
-
Burkholderia pseudomallei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
H2O2 mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.29
-
H2O2 mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
3.8
-
H2O2 mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
5.9
-
H2O2 mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
7.3
-
H2O2 mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
8.2
-
H2O2 mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
8.2
-
H2O2 mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
9.8
-
H2O2 mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
14.4
-
H2O2 wild type enzyme, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
32
-
H2O2 mutant enzyme R108A/W111F/D141A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
34
-
H2O2 mutant enzyme R108A/W111F, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
265
-
H2O2 mutant enzyme D141A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
1190
-
H2O2 mutant enzyme D141N, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
1950
-
H2O2 mutant enzyme R108A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
2110
-
H2O2 mutant enzyme R108K, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
4620
-
H2O2 mutant enzyme R108A/D141A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
5680
-
H2O2 wild type enzyme, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
6140
-
H2O2 mutant enzyme D141E, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei

Cofactor

Cofactor Comment Organism Structure
heme
-
Burkholderia pseudomallei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.32
-
H2O2 mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.39
-
H2O2 mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
0.9
-
H2O2 mutant enzyme R108A/W111F, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
0.9
-
H2O2 mutant enzyme R108A/W111F/D141A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
1.3
-
H2O2 mutant enzyme D141N, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
3.9
-
H2O2 mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
4.4
-
H2O2 mutant enzyme D141A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
8.2
-
H2O2 mutant enzyme D141E, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
12
-
H2O2 mutant enzyme R108K, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
16
-
H2O2 mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
22
-
H2O2 mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
43
-
H2O2 mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
46
-
H2O2 wild type enzyme, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
61
-
H2O2 mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
110
-
H2O2 mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C Burkholderia pseudomallei
140
-
H2O2 mutant enzyme R108A/D141A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
150
-
H2O2 mutant enzyme R108A, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei
1500
-
H2O2 wild type enzyme, catalase activity, at pH 7.0 and 37°C Burkholderia pseudomallei