Crystallization (Comment) | Organism |
---|---|
mutant enzyme D141E | Burkholderia pseudomallei |
Protein Variants | Comment | Organism |
---|---|---|
D141A | the mutant shows decreased catalase and peroxidase activities | Burkholderia pseudomallei |
D141E | the mutant retains normal catalase activity and decreased peroxidase activity | Burkholderia pseudomallei |
D141N | the mutant shows decreased catalase and peroxidase activities | Burkholderia pseudomallei |
R108A | the mutation causes a reduction in catalase activity to 35% of native levels and a decrease in peroxidase activity | Burkholderia pseudomallei |
R108A/D141A | the mutant exhibits near normal catalase activity (82% of native) and decreased peroxidase activity | Burkholderia pseudomallei |
R108A/W111F | the mutant shows stronglydecreased catalase and peroxidase activities | Burkholderia pseudomallei |
R108A/W111F/D141A | the mutant shows strongly decreased catalase and peroxidase activities | Burkholderia pseudomallei |
R108K | the mutant shows decreased catalase and peroxidase activities | Burkholderia pseudomallei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0055 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.0092 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.024 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.03 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.07 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.07 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.09 | - |
H2O2 | mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.12 | - |
H2O2 | mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.14 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | wild type enzyme, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.19 | - |
H2O2 | mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.27 | - |
H2O2 | mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.31 | - |
H2O2 | wild type enzyme, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.41 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.5 | - |
H2O2 | mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.55 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.56 | - |
H2O2 | mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.92 | - |
H2O2 | mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.98 | - |
H2O2 | mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
3.7 | - |
H2O2 | wild type enzyme, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
13 | - |
H2O2 | mutant enzyme R108A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
32 | - |
H2O2 | mutant enzyme R108A/D141A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
35 | - |
H2O2 | mutant enzyme R108A/W111F/D141A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
36 | - |
H2O2 | mutant enzyme R108A/W111F, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
60 | - |
H2O2 | mutant enzyme D141A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
75 | - |
H2O2 | mutant enzyme D141E, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
95 | - |
H2O2 | mutant enzyme D141N, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
180 | - |
H2O2 | mutant enzyme R108K, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia pseudomallei | Q939D2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Burkholderia pseudomallei | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
H2O2 | - |
Burkholderia pseudomallei | O2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
KatG | - |
Burkholderia pseudomallei |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
H2O2 | mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.29 | - |
H2O2 | mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
3.8 | - |
H2O2 | mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
5.9 | - |
H2O2 | mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
7.3 | - |
H2O2 | mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
8.2 | - |
H2O2 | mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
8.2 | - |
H2O2 | mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
9.8 | - |
H2O2 | mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
14.4 | - |
H2O2 | wild type enzyme, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
32 | - |
H2O2 | mutant enzyme R108A/W111F/D141A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
34 | - |
H2O2 | mutant enzyme R108A/W111F, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
265 | - |
H2O2 | mutant enzyme D141A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
1190 | - |
H2O2 | mutant enzyme D141N, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
1950 | - |
H2O2 | mutant enzyme R108A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
2110 | - |
H2O2 | mutant enzyme R108K, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
4620 | - |
H2O2 | mutant enzyme R108A/D141A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
5680 | - |
H2O2 | wild type enzyme, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
6140 | - |
H2O2 | mutant enzyme D141E, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Burkholderia pseudomallei |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
H2O2 | mutant enzyme R108A/W111F/D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.39 | - |
H2O2 | mutant enzyme R108A/W111F, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
0.9 | - |
H2O2 | mutant enzyme R108A/W111F, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
0.9 | - |
H2O2 | mutant enzyme R108A/W111F/D141A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
1.3 | - |
H2O2 | mutant enzyme D141N, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
3.9 | - |
H2O2 | mutant enzyme R108K, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
4.4 | - |
H2O2 | mutant enzyme D141A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
8.2 | - |
H2O2 | mutant enzyme D141E, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
12 | - |
H2O2 | mutant enzyme R108K, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
16 | - |
H2O2 | mutant enzyme R108A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
22 | - |
H2O2 | mutant enzyme R108A/D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
43 | - |
H2O2 | mutant enzyme D141A, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
46 | - |
H2O2 | wild type enzyme, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
61 | - |
H2O2 | mutant enzyme D141E, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
110 | - |
H2O2 | mutant enzyme D141N, peroxidase activity, at pH 4.5 and 25°C | Burkholderia pseudomallei | |
140 | - |
H2O2 | mutant enzyme R108A/D141A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
150 | - |
H2O2 | mutant enzyme R108A, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei | |
1500 | - |
H2O2 | wild type enzyme, catalase activity, at pH 7.0 and 37°C | Burkholderia pseudomallei |