Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Synechococcus sp. |
Crystallization (Comment) | Organism |
---|---|
- |
Burkholderia pseudomallei |
- |
Mycobacterium tuberculosis |
- |
Synechococcus sp. |
Protein Variants | Comment | Organism |
---|---|---|
D141A | the mutant shows 1.5% of wild type catalase activity and 132% of wild type peroxidase activity | Burkholderia pseudomallei |
D141E | the mutant shows 80% of wild type catalase activity and 143% of wild type peroxidase activity | Burkholderia pseudomallei |
D141N | the mutant shows 10% of wild type catalase activity and 123% of wild type peroxidase activity | Burkholderia pseudomallei |
D152N | the mutant shows 2.7% of wild type catalase activity and 234% of wild type peroxidase activity | Synechococcus sp. |
D152S | the mutant shows 5.7% of wild type catalase activity and 237% of wild type peroxidase activity | Synechococcus sp. |
D152W | the mutant shows 0.6% of wild type catalase activity and 672% of wild type peroxidase activity | Synechococcus sp. |
D402E | the mutant shows 0.6% of wild type catalase activity and 65% of wild type peroxidase activity | Synechococcus sp. |
D402N | the mutant shows 0.5% of wild type catalase activity and 56% of wild type peroxidase activity | Synechococcus sp. |
E253D | the mutant shows 42% of wild type catalase activity and 280% of wild type peroxidase activity | Synechococcus sp. |
E253Q | the mutant shows 25% of wild type catalase activity and 96% of wild type peroxidase activity | Synechococcus sp. |
H106C | the mutant shows 0.01% of wild type catalase activity and 1.7% of wild type peroxidase activity | Escherichia coli |
H106Y | the mutant shows 0.008% of wild type catalase activity and 2.7% of wild type peroxidase activity | Escherichia coli |
H108Q | the mutant shows 0.03% of wild type catalase activity and 47% of wild type peroxidase activity | Mycobacterium tuberculosis |
H112A | the mutant shows 0.02% of wild type catalase activity and 2.3% of wild type peroxidase activity | Burkholderia pseudomallei |
H112N | the mutant shows 0.05% of wild type catalase activity and 2.3% of wild type peroxidase activity | Burkholderia pseudomallei |
H123E | the mutant shows 0.03% of wild type catalase activity and 13% of wild type peroxidase activity | Synechococcus sp. |
H123Q | the mutant shows 0.02% of wild type catalase activity and 7.4% of wild type peroxidase activity | Synechococcus sp. |
H257Y | the mutant shows 0.05% of wild type catalase activity and 1.8% of wild type peroxidase activity | Escherichia coli |
H290Q | the mutant shows 0.09% of wild type catalase activity and 5.6% of wild type peroxidase activity | Synechococcus sp. |
I248F | the mutant shows 12% of wild type catalase activity and 124% of wild type peroxidase activity | Synechococcus sp. |
M264A | the mutant shows 0.15% of wild type catalase activity and 160% of wild type peroxidase activity | Burkholderia pseudomallei |
M264L | the mutant shows 0.02% of wild type catalase activity and 140% of wild type peroxidase activity | Burkholderia pseudomallei |
M275I | the mutant shows 0.6% of wild type catalase activity and 640% of wild type peroxidase activity | Synechococcus sp. |
N153A | the mutant shows 6% of wild type catalase activity and 60% of wild type peroxidase activity | Synechococcus sp. |
N153D | the mutant shows 17% of wild type catalase activity and 130% of wild type peroxidase activity | Synechococcus sp. |
N251L | the mutant shows 33% of wild type catalase activity and 415% of wild type peroxidase activity | Synechococcus sp. |
P252A | the mutant shows 110% of wild type catalase activity and 97% of wild type peroxidase activity | Synechococcus sp. |
R102C | the mutant shows 0.5% of wild type catalase activity and 10% of wild type peroxidase activity | Escherichia coli |
R102K | the mutant shows 1.8% of wild type catalase activity and 13% of wild type peroxidase activity | Escherichia coli |
R102L | the mutant shows 1% of wild type catalase activity and 13% of wild type peroxidase activity | Escherichia coli |
R104L | the mutant shows 0.06% of wild type catalase activity and 200% of wild type peroxidase activity | Mycobacterium tuberculosis |
R108A | the mutant shows 31% of wild type catalase activity and 23% of wild type peroxidase activity | Burkholderia pseudomallei |
R108K | the mutant shows 8% of wild type catalase activity and 21% of wild type peroxidase activity | Burkholderia pseudomallei |
R119A | the mutant shows 15% of wild type catalase activity and 12% of wild type peroxidase activity | Synechococcus sp. |
R119N | the mutant shows 0.5% of wild type catalase activity and 5% of wild type peroxidase activity | Synechococcus sp. |
R418L | the mutant shows 0.6% of wild type catalase activity and 192% of wild type peroxidase activity | Mycobacterium tuberculosis |
R426A | the mutant shows 4.3% of wild type catalase activity and 99% of wild type peroxidase activity | Burkholderia pseudomallei |
R426K | the mutant shows 70% of wild type catalase activity and 97% of wild type peroxidase activity | Burkholderia pseudomallei |
R439A | the mutant shows 4.9% of wild type catalase activity and 12% of wild type peroxidase activity | Synechococcus sp. |
R439N | the mutant shows 3.1% of wild type catalase activity and 100% wild type peroxidase activity | Synechococcus sp. |
S315T | the mutant shows 52% of wild type catalase activity and 50% of wild type peroxidase activity | Mycobacterium tuberculosis |
S324T | the mutant shows 109% of wild type catalase activity and 94% of wild type peroxidase activity | Burkholderia pseudomallei |
S335T | the mutant shows 99% of wild type catalase activity and 103% of wild type peroxidase activity | Synechococcus sp. |
W105F | the mutant shows 0.1% of wild type catalase activity and 288% of wild type peroxidase activity | Escherichia coli |
W105L | the mutant shows 0.3% of wild type catalase activity and 197% of wild type peroxidase activity | Escherichia coli |
W111F | the mutant shows 0.05% of wild type catalase activity and 75% of wild type peroxidase activity | Burkholderia pseudomallei |
W122A | the mutant shows no catalase activity and 100% wild type peroxidase activity | Synechococcus sp. |
W122F | the mutant shows no catalase activity and 90% of wild type peroxidase activity | Synechococcus sp. |
W321F | the mutant shows 38% of wild type catalase activity and 18% of wild type peroxidase activity | Mycobacterium tuberculosis |
W341A | the mutant shows 0.5% of wild type catalase activity and 25% of wild type peroxidase activity | Synechococcus sp. |
W341F | the mutant shows 42% of wild type catalase activity and 190% of wild type peroxidase activity | Synechococcus sp. |
Y229F | the mutant shows 0.002% of wild type catalase activity and 1360% of wild type peroxidase activity | Mycobacterium tuberculosis |
Y238A | the mutant shows 0.05% of wild type catalase activity and 140% of wild type peroxidase activity | Burkholderia pseudomallei |
Y238F | the mutant shows 0.15% of wild type catalase activity and 64% of wild type peroxidase activity | Burkholderia pseudomallei |
Y249F | the mutant shows 0.17% of wild type catalase activity and 121% of wild type peroxidase activity | Synechococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia pseudomallei | Q939D2 | - |
- |
Escherichia coli | - |
- |
- |
Mycobacterium tuberculosis | P9WIE5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WIE5 | - |
- |
Synechococcus sp. | Q31MN3 | - |
- |
Synechococcus sp. PCC 7942 | Q31MN3 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
KatG | - |
Escherichia coli |
KatG | - |
Burkholderia pseudomallei |
KatG | - |
Mycobacterium tuberculosis |
KatG | - |
Synechococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
peroxidase activity of KatG | Escherichia coli |
5.5 | - |
peroxidase activity of KatG | Burkholderia pseudomallei |
5.5 | - |
peroxidase activity of KatG | Mycobacterium tuberculosis |
5.5 | - |
peroxidase activity of KatG | Synechococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Escherichia coli | |
heme | - |
Burkholderia pseudomallei | |
heme | - |
Mycobacterium tuberculosis | |
heme | ferric and ferrous KatG show predominant five-coordinate high-spin heme. However, the ferric form shows also small amounts of both six-coordinate high-spin and six-coordinate low-spin, and the ferrous form also exhibits a 6-coordinate low-spin heme | Synechococcus sp. |