Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isoniazid | i.e. isonicotinic acid hydrazide, strong competitive inhibition | Mycolicibacterium smegmatis | |
Isonicotinic acid hydrazide | - |
Mycolicibacterium smegmatis | |
KCN | competitive inhibition against tert-butyl hydroperoxide | Mycolicibacterium smegmatis | |
additional information | not inhibited by 3-amino-1,2,4-triazole, nicotinamide, isonicotinamide or pyrazinamide | Mycolicibacterium smegmatis | |
NaN3 | weak competitive inhibition against o-dianisidine | Mycolicibacterium smegmatis | |
nicotinic acid hydrazide | strong competitive inhibition | Mycolicibacterium smegmatis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) | peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis | |
0.14 | - |
o-Dianisidine | peroxidatic activity, with hydrogen peroxide as cosubstrate, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis | |
0.14 | - |
o-Dianisidine | peroxidatic activity, with tert-butyl hydroperoxide as cosubstrate, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis | |
0.17 | - |
NADPH | peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis | |
0.23 | - |
NADH | peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis | |
0.31 | - |
pyrogallol | peroxidatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis | |
0.6 | - |
H2O2 | catalatic activity, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
4 * 80000, SDS-PAGE | Mycolicibacterium smegmatis |
81889 | - |
4 * 81889, calculated from amino acid sequence | Mycolicibacterium smegmatis |
330000 | - |
gel filtration | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, Superdex 200 gel filtration, Mono Q column chromatography, and phenyl-Superose column chromatography | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
crude extract, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis |
533 | - |
after 214fold purification, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Mycolicibacterium smegmatis | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
3,3'-diaminobenzidine + H2O2 | - |
Mycolicibacterium smegmatis | oxidized 3,3'-diaminobenzidine + H2O | - |
? | |
H2O2 | - |
Mycolicibacterium smegmatis | O2 + H2O | - |
? | |
H2O2 | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | O2 + H2O | - |
? | |
additional information | the enzyme is very slowly reduced by dithionite | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | the enzyme is very slowly reduced by dithionite | Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
NADH + H2O2 | - |
Mycolicibacterium smegmatis | ? | - |
? | |
NADH + H2O2 | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? | - |
? | |
NADPH + H2O2 | - |
Mycolicibacterium smegmatis | ? | - |
? | |
o-dianisidine + H2O2 | - |
Mycolicibacterium smegmatis | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + tert-butyl hydroperoxide | - |
Mycolicibacterium smegmatis | oxidized o-dianisidine + ? | - |
? | |
pyrogallol + H2O2 | - |
Mycolicibacterium smegmatis | ? + H2O | - |
? | |
pyrogallol + H2O2 | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | ? + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 80000, SDS-PAGE | Mycolicibacterium smegmatis |
homotetramer | 4 * 81889, calculated from amino acid sequence | Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | catalase-peroxidase contains 2 heme molecules per tetramer | Mycolicibacterium smegmatis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
KCN | using tert-butyl hydroperoxide as substrate, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis | |
0.0043 | - |
Isonicotinic acid hydrazide | using o-dianisidine as substrate, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis | |
0.0053 | - |
nicotinic acid hydrazide | using o-dianisidine as substrate, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis | |
1.1 | - |
NaN3 | using o-dianisidine as substrate, in 50 mM phosphate buffer, pH 7.0, at 25°C | Mycolicibacterium smegmatis |
General Information | Comment | Organism |
---|---|---|
physiological function | catalase-peroxidase is involved in isoniazid activation | Mycolicibacterium smegmatis |