Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.21 extracted from

  • Kengen, S.W.; Bikker, F.J.; Hagen, W.R.; de Vos, W.M.; van der Oost, J.
    Characterization of a catalase-peroxidase from the hyperthermophilic archaeon Archaeoglobus fulgidus (2001), Extremophiles, 5, 323-332.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-1,2,4-triazole
-
Archaeoglobus fulgidus
azide inhibits the peroxidase activity of the enzyme Archaeoglobus fulgidus
cyanide inhibits the peroxidase activity of the enzyme Archaeoglobus fulgidus
H2O2 at a H2O2 concentration of 1 mM, a half-life of inactivation of approximately 60 s is observed, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) protects the enzyme from inactivation by H2O2 Archaeoglobus fulgidus
hydroxylamine inhibits the peroxidase activity of the enzyme Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.85
-
H2O2 peroxidase activity, 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84900
-
2 * 84900, calculated from amino acid sequence Archaeoglobus fulgidus
85000
-
2 * 85000, SDS-PAGE Archaeoglobus fulgidus
169000
-
gel filtration Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O28050
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
peroxidase activity from crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus
23.3
-
peroxidase activity after 3.17fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus
2375
-
catalase activity after heat treatment of the crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C Archaeoglobus fulgidus
6922
-
catalase activity after 2.91fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Archaeoglobus fulgidus oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
?
2,4-dichlorophenol + H2O2
-
Archaeoglobus fulgidus ?
-
?
3,3'-diaminobenzidine + H2O2
-
Archaeoglobus fulgidus oxidized 3,3'-diaminobenzidine + H2O
-
?
3,3'-dimethoxybenzidine + H2O2
-
Archaeoglobus fulgidus oxidized 3,3'-dimethoxybenzidine + H2O
-
?
4-phenylenediamine + H2O2
-
Archaeoglobus fulgidus oxidized 4-phenylenediamine + H2O
-
?
aminoantipyrine + H2O2
-
Archaeoglobus fulgidus oxidized aminoantipyrine + H2O
-
?
H2O2
-
Archaeoglobus fulgidus O2 + H2O
-
?
additional information no activity is detected with NADH or NADPH Archaeoglobus fulgidus ?
-
?
additional information the catalase activity strongly exceeds the peroxidase activity Archaeoglobus fulgidus ?
-
?
o-dianisidine + H2O2
-
Archaeoglobus fulgidus oxidized o-dianisidine + H2O
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 85000, SDS-PAGE Archaeoglobus fulgidus
homodimer 2 * 84900, calculated from amino acid sequence Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
catalase activity Archaeoglobus fulgidus
80
-
peroxidase activity Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
peroxidase activity Archaeoglobus fulgidus
6
-
catalase activity Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
heme the enzyme possesses 0.25 heme per subunit Archaeoglobus fulgidus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.02
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus hydroxylamine
0.025
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus azide
0.15
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus azide
1
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus cyanide
1
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus cyanide
1.5
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus hydroxylamine
6
-
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus 3-Amino-1,2,4-triazole
30
-
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C Archaeoglobus fulgidus 3-Amino-1,2,4-triazole