Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-Amino-1,2,4-triazole | - |
Archaeoglobus fulgidus | |
azide | inhibits the peroxidase activity of the enzyme | Archaeoglobus fulgidus | |
cyanide | inhibits the peroxidase activity of the enzyme | Archaeoglobus fulgidus | |
H2O2 | at a H2O2 concentration of 1 mM, a half-life of inactivation of approximately 60 s is observed, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) protects the enzyme from inactivation by H2O2 | Archaeoglobus fulgidus | |
hydroxylamine | inhibits the peroxidase activity of the enzyme | Archaeoglobus fulgidus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.85 | - |
H2O2 | peroxidase activity, 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84900 | - |
2 * 84900, calculated from amino acid sequence | Archaeoglobus fulgidus |
85000 | - |
2 * 85000, SDS-PAGE | Archaeoglobus fulgidus |
169000 | - |
gel filtration | Archaeoglobus fulgidus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | O28050 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, hydroxyapatite column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration | Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.45 | - |
peroxidase activity from crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus |
23.3 | - |
peroxidase activity after 3.17fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus |
2375 | - |
catalase activity after heat treatment of the crude extract, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C | Archaeoglobus fulgidus |
6922 | - |
catalase activity after 2.91fold purification, in 50 mM potassium phosphate/sodium citrate buffer, pH 6.0, at 70°C | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2 | - |
Archaeoglobus fulgidus | oxidized 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O | - |
? | |
2,4-dichlorophenol + H2O2 | - |
Archaeoglobus fulgidus | ? | - |
? | |
3,3'-diaminobenzidine + H2O2 | - |
Archaeoglobus fulgidus | oxidized 3,3'-diaminobenzidine + H2O | - |
? | |
3,3'-dimethoxybenzidine + H2O2 | - |
Archaeoglobus fulgidus | oxidized 3,3'-dimethoxybenzidine + H2O | - |
? | |
4-phenylenediamine + H2O2 | - |
Archaeoglobus fulgidus | oxidized 4-phenylenediamine + H2O | - |
? | |
aminoantipyrine + H2O2 | - |
Archaeoglobus fulgidus | oxidized aminoantipyrine + H2O | - |
? | |
H2O2 | - |
Archaeoglobus fulgidus | O2 + H2O | - |
? | |
additional information | no activity is detected with NADH or NADPH | Archaeoglobus fulgidus | ? | - |
? | |
additional information | the catalase activity strongly exceeds the peroxidase activity | Archaeoglobus fulgidus | ? | - |
? | |
o-dianisidine + H2O2 | - |
Archaeoglobus fulgidus | oxidized o-dianisidine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 85000, SDS-PAGE | Archaeoglobus fulgidus |
homodimer | 2 * 84900, calculated from amino acid sequence | Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
catalase activity | Archaeoglobus fulgidus |
80 | - |
peroxidase activity | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
peroxidase activity | Archaeoglobus fulgidus |
6 | - |
catalase activity | Archaeoglobus fulgidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | the enzyme possesses 0.25 heme per subunit | Archaeoglobus fulgidus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.02 | - |
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus | hydroxylamine | |
0.025 | - |
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus | azide | |
0.15 | - |
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus | azide | |
1 | - |
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus | cyanide | |
1 | - |
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus | cyanide | |
1.5 | - |
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus | hydroxylamine | |
6 | - |
catalase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus | 3-Amino-1,2,4-triazole | |
30 | - |
peroxidase activity, in 50 mM potassium phosphate/sodium citrate buffer, pH 4.5, at 80°C | Archaeoglobus fulgidus | 3-Amino-1,2,4-triazole |