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Literature summary for 1.11.1.21 extracted from

  • Chouchane, S.; Lippai, I.; Magliozzo, R.S.
    Catalase-peroxidase (Mycobacterium tuberculosis KatG) catalysis and isoniazid activation (2000), Biochemistry, 39, 9975-9983.
    View publication on PubMed
Search for more literature for 1.11.1.21

Application

Application Comment Organism
medicine KatG is responsible for activation of the antituberculosis antibiotic isoniazid (isonicotinic acid hydrazide) Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain UM262 Mycobacterium tuberculosis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
81000
-
 2 * 81000, SDS-PAGE Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.95
-
 peroxidase activity, in 50 mM sodium acetate buffer, pH 5.5, at 25°C Mycobacterium tuberculosis
4500
-
 catalase activity, in phosphate buffer, pH 7.5, at 25°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2O2
-
 Mycobacterium tuberculosis O2 + H2O
-
 ?
o-dianisidine + H2O2
-
 Mycobacterium tuberculosis oxidized o-dianisidine + H2O
-
 ?
tert-butyl hydroperoxide + H2O2
-
 Mycobacterium tuberculosis oxidized tert-butyl hydroperoxide + H2O
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 81000, SDS-PAGE Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
KatG
-
 Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
heme KatG contains 1 equivalent of heme per dimer Mycobacterium tuberculosis